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| Status |
Public on Nov 14, 2019 |
| Title |
Transcriptional changes involved in inhibition of biofilm formation by ε-Polylysine in Salmonella Typhimurium |
| Platform organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
| Sample organism |
Salmonella enterica subsp. enterica serovar Typhimurium |
| Experiment type |
Expression profiling by array
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| Summary |
Salmonella Typhimurium is one of the major foodborne pathogens due to its biofilm formation on food contact surfaces. A polymer of positively charged lysine, ε-Polylysine has been demonstrated to inhibit biofilm formation of both Gram-positive and -negative bacteria. To elucidate the mechanism for inhibition of biofilm formation by ε-Polylysine, transcriptional profiles were compared between the cells before and after treatment with ε-Polylysine in Salmonella Typhimurium. A genome-wide DNA microarray analysis was performed after cultivation in 0.1% bacto soytone in the presence of 0.001% ε-Polylysine at 30°C for 2 h. Genes involved in curli and cellulose production, quorum sensing, and flagellar motility were down-regulated, whereas genes associated with colanic acid synthesis were up-regulated. The data from microarray was validated by RT-qPCR. Furthermore, production of colanic acid in S. Typhimurium decreased in the presence of ε-Polylysine. The outcome of this study provides a basic understanding of the anti-biofilm mechanisms of ε-Polylysine and may contributes to develop new disinfectant to control biofilm during food manufacturing and storage.
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| Overall design |
Gene expression during initial adhension (2 hours) were measured with or withour exposure to 0.001% ε-Polylysine. Three dependent experiments were performed at each time.
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| Contributor(s) |
Shen C, Islam T, Masuda Y, Honjoh K, Miyamoto T |
| Citation missing |
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| Submission date |
Nov 13, 2019 |
| Last update date |
Nov 16, 2019 |
| Contact name |
Cunkuan Shen |
| E-mail(s) |
sck12341@gmail.com
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| Phone |
09013614282
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| Organization name |
kyushu university
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| Street address |
Motooka
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| City |
fukuoka nishi ward |
| State/province |
fukuoka |
| ZIP/Postal code |
8190385 |
| Country |
Japan |
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| Platforms (1) |
| GPL27746 |
Agilent-045986 Salmonella Typhimurium LT2 |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA589476 |
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