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Series GSE140408 Query DataSets for GSE140408
Status Public on Jan 09, 2020
Title The influence of acetate in the medium to the global gene expression of C. glutamicum wild type (WT) and the C. glutamicum ΔcyaB mutant (ΔcyaB)
Organism Corynebacterium glutamicum ATCC 13032
Experiment type Expression profiling by array
Summary The cAMP-dependent transcriptional regulator GlxR serves as a central hub in the regulatory network of the actinobacterial model organism Corynebacterium glutamicum and controls expression of ~10% of all genes. The consequences of a lowered cAMP level are mostly unkown. A single gene (cyaB) for a cAMP-synthesizing adenylate cyclase was identified and it had been reported that a cyaB mutant grows like the wild type on glucose, but has a strong growth defect in the presence of acetate. Our work aimed to understand the molecular basis for the acetate sensitivity of a ΔcyaB mutant and improve our knowledge on the physiological function of cAMP. Our results indicate that a diminished expression of the GlxR-activated genes for the cytochrome bc1-aa3 supercomplex in the ΔcyaB mutant causes a reduced capacity to build up membrane potential. This deficiency becomes evident in the presence of acetate or the protonophore CCCP, which increase the proton permeability of the cytoplasmic membrane, and has systemic effects on transport processes including oxidative phosphorylation. The acetate sensitivity of the ΔcyaB mutant could be abolished by a single amino acid exchange in GlxR. Our results demonstrate a key role of cAMP and GlxR in the control of energy metabolism in corynebacteria.
 
Overall design DNA microarray analyses of the ΔcyaB mutant and the wild type (WT), grown in minimal medium with glucose and acetate (100 mM each) were performed. For this purpose RNA was isolated from exponentially growing cells cultivated in CGXII medium containing glucose and acetate (each 100 mM) as carbon source. Three biological replicates of both strains were performed.
 
Contributor(s) Wolf N, Bussmann M, Koch-Koerfges A, Katcharava N, Schulte J, Polen T, Hartl J, Vorholt JA, Baumgart M, Bott M
Citation(s) 32117117
Submission date Nov 14, 2019
Last update date Apr 14, 2021
Contact name Tino Polen
E-mail(s) t.polen@fz-juelich.de
Organization name Forschungszentrum Jülich GmbH
Department IBG-1: Biotechnology
Street address Leo Brandt Str.
City Juelich
State/province NRW
ZIP/Postal code 52425
Country Germany
 
Platforms (1)
GPL9860 FZ_IBT1_Cglutamicum_10K_ver2
Samples (3)
GSM4160794 DcyaB_vs_WT_Glc-Ac_rep1
GSM4160795 DcyaB_vs_WT_Glc-Ac_rep2
GSM4160796 DcyaB_vs_WT_Glc-Ac_rep3
Relations
BioProject PRJNA589619

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140408_DcyaB_vs_WT_Glucose-Acetate_Matrix.xlsx 703.3 Kb (ftp)(http) XLSX
GSE140408_RAW.tar 3.0 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
Processed data are available on Series record

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