|
| Status |
Public on Aug 16, 2020 |
| Title |
Bronchial Epithelial cells gene expression profiles in response to Mycobacterium abscessus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bronchial Epithelial cells were exposed to microparticles generated from mycobacterium abscessus MAB cell wall per an in-house protocol. Control group was exposed to saline. Differentially expressed genes related to MAB cell wall particles were determined.
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| |
|
| Overall design |
Lung on membrane model was developed using human bronchial epithelial cells and lung small vessels endothelial cells. Air liquid interface (ALI) challenged with MAB microparticles (5 samples). Controls challenged with saline (5 samples). Samples were collected after 48 hours. RNA extracted and RNASeq was performed.
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| |
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| Contributor(s) |
Griswold AJ, Mirsaeidi M |
| Citation(s) |
31921151 |
| |
| Submission date |
Nov 15, 2019 |
| Last update date |
Aug 16, 2020 |
| Contact name |
Anthony Griswold |
| E-mail(s) |
agriswold@med.miami.edu
|
| Organization name |
University of Miami
|
| Street address |
1501 NW 10th Ave, BRB 318
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE140439 |
Mycobacterium abscessus -bronchial epithelial cells cross-talk through Type I interferon signaling |
|
| Relations |
| BioProject |
PRJNA589745 |
| SRA |
SRP230179 |
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