|
| Status |
Public on Nov 19, 2019 |
| Title |
RNAseq for the identification of genes induced upon TGR5 stimulation in intestinal stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Transcriptomic exploration for the identification of genes induced upon TGR5 stimulation in intestinal stem cells
Methods: For each biological replicate, GFPhi cells were isolated by FACS from intestines from 4 pooled Lgr5-eGFP-IRES-CreERT2 mice. About 200.000 GFPhi cells were then embedded in Matrigel (20.000 per well in 10µL Matrigel drop) and after 4 hours were treated with INT-777 (30µM) or DMSO as control. After 16 hours RNA was extracted (RNAqueous Kit, Thermofisher) for RNAseq analysis. This experiment was repeated 3 times in 3 different days (n=3 biological replicates).
Results: INT-777 treatment results in the activation of a panel of genes showing high enrichment of TEADs family of TF in associated motifs.
|
| |
|
| Overall design |
Design: For each biological replicate, GFPhi cells were isolated by FACS from intestines from 4 pooled Lgr5-eGFP-IRES-CreERT2 mice. About 200.000 GFPhi cells were then embedded in Matrigel (20.000 per well in 10µL Matrigel drop) and after 4 hours were treated with INT-777 (30µM) or DMSO as control. After 16 hours RNA was extracted (RNAqueous Kit, Thermofisher) for RNAseq analysis. This experiment was repeated 3 times in 3 different days (n=3 biological replicates).
|
| |
|
| Contributor(s) |
El Alam G, Sorrentino G |
| Citation(s) |
32485177 |
| |
| Submission date |
Nov 18, 2019 |
| Last update date |
Oct 23, 2023 |
| Contact name |
Gaby El Alam |
| E-mail(s) |
gaby.elalam@epfl.ch
|
| Organization name |
École polytechnique fédérale de Lausanne
|
| Department |
SV LISP
|
| Lab |
Laboratory of Integrative Systems Physiology
|
| Street address |
Station 15
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA590174 |
| SRA |
SRP230410 |
Less...
More...