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| Status |
Public on Jun 18, 2020 |
| Title |
Heat-shock molecular chaperone Hsp90 is involved in heterochromatin assembly in Schizosaccharomyces pombe. |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Here, we report the high-throughput profiling of histone modification (H3K9me2) in fission yeast Schizosaccharomyces pombe. We generated genome-wide H3K9me2 maps of fission yeast mutants in swo1-26 (temperature sensitive, ts) cells at 25℃ and 37℃. We find that H3K9me2 enrichment at heterochromatin regions, especially at the mating-type locus and subtelomeres, is compromised, suggesting heterochromatin assembly defects.
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| Overall design |
Examination of H3K9me2 in wt, ago1D and tas3D growing at 25℃. Particularly, swo1-26 mutant cells are cultured under permissive condition (25℃) and non-permissive condition (25℃ shifted to 37℃ for 4 h).
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| Contributor(s) |
Li W, Sun L, Jin Q |
| Citation(s) |
32499408 |
| |
| Submission date |
Nov 18, 2019 |
| Last update date |
Sep 17, 2020 |
| Contact name |
Li Sun |
| E-mail(s) |
sunli@stu.xmu.edu.cn
|
| Organization name |
Xiamen University
|
| Street address |
South Xiang'an Road
|
| City |
Xiamen |
| State/province |
Fujian |
| ZIP/Postal code |
361102 |
| Country |
China |
| |
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| Platforms (1) |
| GPL22668 |
Ion Torrent PGM (Schizosaccharomyces pombe) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA590253 |
| SRA |
SRP230449 |
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