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| Status |
Public on Feb 03, 2020 |
| Title |
The transcriptional responses of C. glutamicum to the secretory production of two different heterologous proteins |
| Organism |
Corynebacterium glutamicum ATCC 13032 |
| Experiment type |
Expression profiling by array
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| Summary |
High level secretion of proteins via the Sec protein export pathway leads to secretion stress, a phenomenon that is thought to be caused by the accumulation of incompletely or misfolded proteins at the membrane-cell envelope interface. We have analyzed the transcriptional responses of C. glutamicum to the secretory production of two different heterologous proteins and found that, in both cases, the expression of the gene encoding a homologue of the extracytosolic HtrA protease was highly upregulated.
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| Overall design |
Transcriptional responses of C. glutamicum to the secretory production of heterologous proteins were determined in 3 sets. Set 1 comprises 3 biological replicates of C.glutamicum WT carrying plasmid for expression of AmyE without signal peptide. Set 2 comprises 3 biological replicates of C.glutamicum WT carrying plasmid for expression of AmyE fused with signal peptide. Set 3 comprises 3 biological replicates of C.glutamicum WT carrying plasmid for expression of cutinase fused with NprE signal peptide. In all sets/replicates the induced culture was compared to the uninduced culture.
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| Contributor(s) |
Jurischka S, Polen T, Freudl R |
| Citation(s) |
31964372 |
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| Submission date |
Nov 20, 2019 |
| Last update date |
Apr 14, 2021 |
| Contact name |
Tino Polen |
| E-mail(s) |
t.polen@fz-juelich.de
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| Organization name |
Forschungszentrum Jülich GmbH
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| Department |
IBG-1: Biotechnology
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| Street address |
Leo Brandt Str.
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| City |
Juelich |
| State/province |
NRW |
| ZIP/Postal code |
52425 |
| Country |
Germany |
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| Platforms (1) |
| GPL9860 |
FZ_IBT1_Cglutamicum_10K_ver2 |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA590712 |
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