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Series GSE140837 Query DataSets for GSE140837
Status Public on Mar 06, 2020
Title Next Generation Sequencing analysis of the effects of seed HSF (HaHSFA9 or HSFA4a) overexpression in transgenic tobacco
Organism Nicotiana tabacum
Experiment type Expression profiling by high throughput sequencing
Summary Background: Heat Stress Factor A9 (A9), a seed-specific transcription factor contributing to seed longevity, also enhances phytochrome-dependent seedling greening
Results: The RNA-seq analyses of imbibed-seed transcripts here reported indicated, potential, additional effects of A9 on cryptochrome-mediated blue-light responses. These analyses also suggested that in contrast to the A9 effects on longevity, which require coactivation by additional factors as A4a, A9 alone might suffice for the enhancement of photomorphogenesis at the seedling stage. We find that upon its seed-specific over-expression, A9 indeed enhanced the expected blue-light responses. Comparative loss-of-function analyses of longevity and greening, performed by similar expression of dominant-negative and inactive forms of A9, not only confirmed the additional greening effects of A9, but also were consistent with A9 not requiring A4a (or additional factors) for the greening effects
Conclusion: Our results strongly indicate that A9 would differentially regulate seed longevity and photomorphogenesis at the seedling stage, A9 alone sufficing for both the phytochrome- and cryptochrome-dependent greening enhancement effects
 
Overall design Transcriptome profiling and differential expression analyses between samples of transgenic and non-transgenic Nicotiana tabacum lines
 
Contributor(s) Almoguera C, Prieto-Dapena P, Carranco R, Ruiz JL, Jordano J
Citation(s) 32155706
Submission date Nov 22, 2019
Last update date Mar 13, 2020
Contact name Jose Luis Ruiz Rodriguez
Organization name IPBLN-CSIC
Street address Avda. del Conocimiento 17. P. T. Ciencias de la Salud
City Granada
State/province Granada
ZIP/Postal code 18016
Country Spain
 
Platforms (1)
GPL25653 Illumina HiSeq 4000 (Nicotiana tabacum)
Samples (6)
GSM4188537 DS10:A9 transgenic seeds
GSM4188538 NT non-transgenic seeds
GSM4188539 DS10:A4a transgenic seeds
Relations
BioProject PRJNA591121
SRA SRP231425

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140837_Table_S1.xls.gz 866.6 Kb (ftp)(http) XLS
GSE140837_Table_S3.xls.gz 1.2 Mb (ftp)(http) XLS
GSE140837_Table_S4.xls.gz 337.2 Kb (ftp)(http) XLS
GSE140837_Table_S5.xls.gz 1.4 Mb (ftp)(http) XLS
GSE140837_Table_S6.xls.gz 329.9 Kb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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