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| Status |
Public on Oct 26, 2021 |
| Title |
K27M and K36M mutations on non canonical Histone 3.3 promote redistribution of antagonistic chromatin marks, disrupted development, and tumorigenesis |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We conducted RNA-seq analysis to identify genes that are differentially expressed in H3K27M and H3K36M oncohistones compared to wt and control eye tissue (eye discs), followed by smRNA-seq profiling based on our results and to confirm upregulation of piRNAs
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| Overall design |
3 data types (RNA-seq, smRNA-seq and ChIP-seq data). RNA-seq and smRNA-seq experiments has 12 samples, which is composed of 3 replicates of 4 genotypes (H3K27M, H3K36M, histone WT and control eye tissue (Yw)). Additional RNA-seq RNAi experiments has been performed on 10 sets (3 replicates each, 30 samples total). H3K27me3 and H3K36me2 ChIP-seq was also performed (14 samples)
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| Contributor(s) |
Chaouch A, Berlandi J, Frey F, Chen CC, Badini S, Marchione D, Garcia B, Harutyunyan A, Krug B, Chen H, Majewski J, Hébert S, Jeibmann A, Kleinman CL, Hasselblatt M, Lasko P, Shirinian M, Jabado N |
| Citation(s) |
34739871 |
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| Submission date |
Nov 25, 2019 |
| Last update date |
Jan 25, 2022 |
| Contact name |
Claudia L Kleinman |
| E-mail(s) |
claudia.kleinman@mcgill.ca
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| Phone |
514-340-8222 25139
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| Organization name |
Lady Davis Institute for Medical Research
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| Department |
Human Genetics
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| Street address |
3999 Côte Ste-Catherine Road
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| City |
Montréal |
| State/province |
Québec |
| ZIP/Postal code |
H3T 1E2 |
| Country |
Canada |
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| Platforms (2) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (68)
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| Relations |
| BioProject |
PRJNA591601 |
| SRA |
SRP233209 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE140979_RAW.tar |
5.3 Gb |
(http)(custom) |
TAR (of BEDGRAPH, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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