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Status |
Public on Nov 26, 2019 |
Title |
Phenotypic and Transcriptomic Analysis of Two Pinellia ternata Varieties T2 line and T2Plus line |
Organism |
Pinellia ternata |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Pinellia (Pinellia ternata (Thunb.) Breit.), as important medicinal plant, has been used to treat various ailments for a long time. The sixteen ploid plant (2n=16*13=208) Pinellia T2Plus line was obtained from an octoploid (2n=8*13=104) T2 line by chromosome-doubling technique. Compared with T2 line, the content of various medicinal components (polysaccharide, guanosine, adenosine and ephedrine) was increased in T2Plus line. In this study, the transcriptome of T2 line and T2Plus line were characterized by RNA sequencing (RNA-seq) technology. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis on differential expressed unigenes (DEGs) revealed that multiple metabolic pathway were enriched significantly, such as ‘Starch and sucrose metabolism’, ‘Purine metabolism’, ‘Photosynthesis’ and six transcription factors (MYB, WRKY, bHLH, LBD lateral organ boundaries domain (LBD), HD-ZIP homeodomain-zipper (HD-ZIP) and ERF Ethylene-responsive factor (ERF) ) play a key role in difference of transcriptome between T2 line and T2Plus line. These metabolic pathways and transcription factors may play an important role in the difference of medicinal components and epigenetic features between these two Pinellia cultivars. This conclusion provides a robust theoretical basis for the mechanism of the formation of medicinal ingredients in Pinellia cultivars.
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Overall design |
The transcriptome of Pinellia ternata (Thunb.) Breit. T2P line(2n=16*13=208) and T2 line(2n=8*13=104)
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Contributor(s) |
Chen J, Lu J |
Citation(s) |
32165650 |
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Submission date |
Nov 25, 2019 |
Last update date |
Mar 30, 2020 |
Contact name |
Chen Jishuang |
E-mail(s) |
biochenjs@njtech.edu.cn
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Organization name |
Nanjing Tech University
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Department |
College of Biotechnology and Pharmaceutical Engineering
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Street address |
NO.30 Puzhu Road(S)
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City |
Nanjing |
State/province |
Jiangsu |
ZIP/Postal code |
211800 |
Country |
China |
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Platforms (1) |
GPL27806 |
Illumina HiSeq 4000 (Pinellia ternata) |
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Samples (6)
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Relations |
BioProject |
PRJNA591634 |
SRA |
SRP233223 |
Supplementary file |
Size |
Download |
File type/resource |
GSE140998_Trinity.okay.fasta.gz |
24.5 Mb |
(ftp)(http) |
FASTA |
GSE140998_annotation_report.xls.gz |
6.9 Mb |
(ftp)(http) |
XLS |
GSE140998_transcript_exp.matrix.xls.gz |
2.5 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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