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Series GSE140998 Query DataSets for GSE140998
Status Public on Nov 26, 2019
Title Phenotypic and Transcriptomic Analysis of Two Pinellia ternata Varieties T2 line and T2Plus line
Organism Pinellia ternata
Experiment type Expression profiling by high throughput sequencing
Summary Pinellia (Pinellia ternata (Thunb.) Breit.), as important medicinal plant, has been used to treat various ailments for a long time. The sixteen ploid plant (2n=16*13=208) Pinellia T2Plus line was obtained from an octoploid (2n=8*13=104) T2 line by chromosome-doubling technique. Compared with T2 line, the content of various medicinal components (polysaccharide, guanosine, adenosine and ephedrine) was increased in T2Plus line. In this study, the transcriptome of T2 line and T2Plus line were characterized by RNA sequencing (RNA-seq) technology. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis on differential expressed unigenes (DEGs) revealed that multiple metabolic pathway were enriched significantly, such as ‘Starch and sucrose metabolism’, ‘Purine metabolism’, ‘Photosynthesis’ and six transcription factors (MYB, WRKY, bHLH, LBD lateral organ boundaries domain (LBD), HD-ZIP homeodomain-zipper (HD-ZIP) and ERF Ethylene-responsive factor (ERF) ) play a key role in difference of transcriptome between T2 line and T2Plus line. These metabolic pathways and transcription factors may play an important role in the difference of medicinal components and epigenetic features between these two Pinellia cultivars. This conclusion provides a robust theoretical basis for the mechanism of the formation of medicinal ingredients in Pinellia cultivars.
 
Overall design The transcriptome of Pinellia ternata (Thunb.) Breit. T2P line(2n=16*13=208) and T2 line(2n=8*13=104)
 
Contributor(s) Chen J, Lu J
Citation(s) 32165650
Submission date Nov 25, 2019
Last update date Mar 30, 2020
Contact name Chen Jishuang
E-mail(s) biochenjs@njtech.edu.cn
Organization name Nanjing Tech University
Department College of Biotechnology and Pharmaceutical Engineering
Street address NO.30 Puzhu Road(S)
City Nanjing
State/province Jiangsu
ZIP/Postal code 211800
Country China
 
Platforms (1)
GPL27806 Illumina HiSeq 4000 (Pinellia ternata)
Samples (6)
GSM4192150 T2-1
GSM4192151 T2-2
GSM4192152 T2-3
Relations
BioProject PRJNA591634
SRA SRP233223

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Supplementary file Size Download File type/resource
GSE140998_Trinity.okay.fasta.gz 24.5 Mb (ftp)(http) FASTA
GSE140998_annotation_report.xls.gz 6.9 Mb (ftp)(http) XLS
GSE140998_transcript_exp.matrix.xls.gz 2.5 Mb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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