 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 30, 2008 |
| Title |
Gene expression change caused by CYS4 alleles |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Each strain was grown overnight then diluted in fresh YPD to 10% v/v and grown for 3 hours at 30'C. Total RNA was isolated using an RNeasy mini kit, reverse transcribed, labeled with either Cy3 or Cy5, and hybridized to epoxy-coated slides with 6388, 70mer oligos (Qiagen-Operon). After an overnight hybridization, microarrays were scanned using a ScanArray Express laser scanner.
|
| |
|
| Overall design |
Reference, a pool of total RNA from all samples, was used to contrast with each sample. Dye-swap was performed for one of the three replicates of each strain, where Cy3 instead of Cy5 was used to label the reference RNA.
|
| |
|
| Contributor(s) |
Kim H, Fay JC |
| Citation(s) |
19456872 |
| |
| Submission date |
Dec 22, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Hyun Seok Kim |
| E-mail(s) |
Hyun.Kim@UTsouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75063 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7882 |
Washington University Saccharomyces 70 mer 13K oligonucleotide microarray v1 |
|
| Samples (9)
|
| GSM353969 |
Strain M22-cys4-, YPD media, replicate 1 |
| GSM353970 |
Strain M22-cys4-, YPD media, replicate 2 |
| GSM353971 |
Strain M22-cys4-, YPD media, replicate 3 |
| GSM353972 |
Strain M22-cys4::N123I, YPD media, replicate 1 |
| GSM353973 |
Strain M22-cys4::N123I, YPD media, replicate 2 |
| GSM353974 |
Strain M22-cys4::N123I, YPD media, replicate 3 |
|
| Relations |
| BioProject |
PRJNA111189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14102_RAW.tar |
15.1 Mb |
(http)(custom) |
TAR (of CSV) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...