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Status |
Public on Dec 30, 2008 |
Title |
Gene expression change caused by CYS4 alleles |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by array
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Summary |
Each strain was grown overnight then diluted in fresh YPD to 10% v/v and grown for 3 hours at 30'C. Total RNA was isolated using an RNeasy mini kit, reverse transcribed, labeled with either Cy3 or Cy5, and hybridized to epoxy-coated slides with 6388, 70mer oligos (Qiagen-Operon). After an overnight hybridization, microarrays were scanned using a ScanArray Express laser scanner.
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Overall design |
Reference, a pool of total RNA from all samples, was used to contrast with each sample. Dye-swap was performed for one of the three replicates of each strain, where Cy3 instead of Cy5 was used to label the reference RNA.
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Contributor(s) |
Kim H, Fay JC |
Citation(s) |
19456872 |
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Submission date |
Dec 22, 2008 |
Last update date |
Mar 20, 2012 |
Contact name |
Hyun Seok Kim |
E-mail(s) |
Hyun.Kim@UTsouthwestern.edu
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Organization name |
UT Southwestern Medical Center
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Street address |
Harry Hines Blvd.
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City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75063 |
Country |
USA |
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Platforms (1) |
GPL7882 |
Washington University Saccharomyces 70 mer 13K oligonucleotide microarray v1 |
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Samples (9)
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GSM353969 |
Strain M22-cys4-, YPD media, replicate 1 |
GSM353970 |
Strain M22-cys4-, YPD media, replicate 2 |
GSM353971 |
Strain M22-cys4-, YPD media, replicate 3 |
GSM353972 |
Strain M22-cys4::N123I, YPD media, replicate 1 |
GSM353973 |
Strain M22-cys4::N123I, YPD media, replicate 2 |
GSM353974 |
Strain M22-cys4::N123I, YPD media, replicate 3 |
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Relations |
BioProject |
PRJNA111189 |
Supplementary file |
Size |
Download |
File type/resource |
GSE14102_RAW.tar |
15.1 Mb |
(http)(custom) |
TAR (of CSV) |
Processed data included within Sample table |
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