|
| Status |
Public on Jul 21, 2021 |
| Title |
The role of Vitamin C and TET dioxygenases in genome-wide features of regulatory T cells [CMS-IP] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
TET enzymes are essential for the stability and function of regulatory T cells (Tregs), which maintain immune homeostasis and self-tolerance and express the lineage-determining transcription factor Foxp3. We previously showed that Vitamin C acts through TET enzymes to maintain the demethylated status of CNS2, a key intronic enhancer of the Foxp3 gene that is essential for the stability of Foxp3 expression both in vivo and in vitro. Here we show that Vitamin C enables genome-wide features of “induced” T regulatory cells (iTregs) in vitro that overlap with those induced by TET proteins during Treg differentiation in vivo. Vitamin C enhances IL-2 responsiveness in iTregs by increasing phospho-STAT5 levels, STAT5 occupancy and DNA demethylation at key Treg-specific enhancers, and maintains the stable expression of Treg-specific genes including Foxp3 and Il2ra. Our data will be relevant to future studies of the association between plasma Vitamin C levels, Treg function and autoimmunity in humans.
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| |
|
| Overall design |
RNA-seq, ATAC-seq, CMS-IP (5hmC mapping) and WGBS datasets
|
| |
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| Contributor(s) |
Yue X, Samaniego Castruita D, Barwick B, Rao A |
| Citation(s) |
34288360 |
| |
| Submission date |
Nov 28, 2019 |
| Last update date |
Oct 20, 2021 |
| Contact name |
Anjana Rao |
| E-mail(s) |
arao@lji.org
|
| Organization name |
La Jolla Institute for Allergy and Immunology
|
| Department |
Signaling and Gene Expression
|
| Lab |
Anjana Rao
|
| Street address |
9420 Athena Cir
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (16)
|
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| This SubSeries is part of SuperSeries: |
| GSE141152 |
The role of Vitamin C and TET dioxygenases in genome-wide features of regulatory T cells |
|
| Relations |
| BioProject |
PRJNA592358 |
| SRA |
SRP233664 |
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