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| Status |
Public on Jul 24, 2020 |
| Title |
A single cell transcriptome atlas of myeloid blood cell lineages in Drosophila [scRNA-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The Drosophila lymph gland (LG) is the larval hematopoietic organ comprised of multipotent prohemocytes and mature hemocytes and has been a valuable model for understanding mechanisms underlying the hematopoiesis and immune responses. There are three types of mature hemocytes in the lymph gland; plasmatocytes, lamellocytes, and crystal cells, all of which are analogous to myeloid-lineage blood cells. To date, the Drosophila hematopoietic system has been primarily defined by classical genetic methods that may limit the promise of its advantage as a model. In this study, we used single-cell RNA sequencing method to comprehensively profile the heterogeneity of developing myeloid hemocytes in the wild-type lymph gland and their transitions upon active immunity caused by wasp infestation. Moreover, we compared hemocytes originated from the embryonic and the lymph gland hematopoiesis and unraveled genetic and cellular diversity of two different ancestries. Finally, hemocytes of the Drosophila lymph gland and human immune cells are contrasted at a transcriptome level, highlighting evolutionary conservations of myeloid cells across species. Overall, our single-cell RNA seq analyses disclose the development of myeloid hemocytes at a single-cell level and provide comparative insights into two different lineages of hemocytes in Drosophila and perspectives on the evolution of myeloid cells, serving as an ample resource for revealing essentials of the hematopoiesis.
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| Overall design |
Single-cell RNA-sequencing of Drosophila larvae LGs or circulating hemocytes under normal or wasp infested states using Drop-seq
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| Contributor(s) |
Yoon S, Cho B, Oh S, Moon SJ, Nam J, Shim J |
| Citation(s) |
32900993 |
| |
| Submission date |
Dec 02, 2019 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Jin-Wu Nam |
| E-mail(s) |
jwnam@hanyang.ac.kr
|
| Phone |
+82 2-2220-2428
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| Organization name |
Hanyang University
|
| Department |
Department of Life Science
|
| Street address |
Seongdong-Gu Hangdang-dong
|
| City |
Seoul |
| ZIP/Postal code |
133-791 |
| Country |
South Korea |
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| Platforms (1) |
| GPL22106 |
NextSeq 550 (Drosophila melanogaster) |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE141275 |
A single cell transcriptome atlas of myeloid blood cell lineages in Drosophila |
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| Relations |
| BioProject |
PRJNA592992 |
| SRA |
SRP234423 |
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