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| Status |
Public on Aug 25, 2020 |
| Title |
Gene expression analysis in Tibialis anterior (TA) muscle upon titration of myonuclear numbers |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Skeletal muscle myofibers accrue hundreds of nuclei during post-natal development via fusion with activated satellite cells (myoblasts), which is absolutely reliant on expression of the muscle fusogen myomaker (Mymk) in the myoblasts. Using an inducible genetic approach to render myoblasts non-fusogenic (by tamoxifen-inducible Pax7-CreER mediated recombination of the Mymk gene exclusively in satellite cells), we blocked myonuclear accrual at different time-points of post-natal development and thereby titrated the number of nuclei in resultant mutant myofibers. These Microarray assays were carried out on age day 28 (P28) using total RNA isolated from control and mutant muscle to determine changes in transcriptional profiles of these muscles to (a) assess effects of myonuclear titration, and (b) identify adaptive mechanisms elicited in mutant muscles in response to myonuclear deficiency.
We used microarrays to generate information about transcriptional profiles in muscles with varying levels of myonuclear deficiencies.
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| Overall design |
TA muscle was isolated from day 28 (P28) old animals that had either full complement of myonuclei or had received tamoxifen on day 0 (D1w) or day 6 (D2w) to block myoblast fusion and therefore, accrue different myonuclei numbers.
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| Contributor(s) |
Cramer AW, Prasad V, Millay DP |
| Citation(s) |
33293533 |
| |
| Submission date |
Dec 02, 2019 |
| Last update date |
Dec 10, 2020 |
| Contact name |
Douglas Millay |
| E-mail(s) |
Douglas.Millay@cchmc.org
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Street address |
240 Albert Sabin Way MLC 7020
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA593018 |
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