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Series GSE141674

Query DataSets for GSE141674

Status

Public on Dec 10, 2019

Title

Intake of protein modulate immune function, POMC processing and IGF signaling gene expression pathways in peripheral mononuclear cells in home-dwelling old subjects - a transcriptomic approach

Organism

Experiment type

Expression profiling by array

Summary

Adequate intake of protein among older adults is associated with better health outcomes, but conflicting results makes it difficult to define the optimal intake. To further understand the impact of protein intake on metabolic processes we studied genome-wide gene expression changes in peripheral blood mononuclear cells (PBMCs) in home-dwelling old subjects after increased protein intake for 12 weeks.

Overall design

This study was a parallel design double blind randomized controlled intervention study. In total fifty home-dwelling men and women with reduced physical strength and/or performance (≥ 70 yrs) were recrutied to the study. Subjects were stratified by gender and smoking into two groups, receiving either a protein-enriched milk (2 x 20 g protein/d) or an isocaloric carbohydrate drink for breakfast and evening meal for 12 weeks. The test drinks were provided by TINE SA and were identical in color, labeling and appearance, and was blinded to both the study participants and the study staff. Participants maintained their normal diet and physical activity levels during the study period. PBMCs were isolated before and after the intervention. mRNA was extracted using RNeasy Mini Kit (Qiagen) and the RNA quantity and quality was meassured using NanoDrop-1000 (NanoDrop Technologies, Inc., Delaware, USA), and Aglient 2100 Bioanalyzer (Agilent Technologies, Inc., California, USA), resepctilvely. Microarray analysis were performed using Illumina technology. Thirty-six subjects finished the study, and five samples were excluded from the final analysis, leaving us with n=14 in the protein group and n=17 in the carbohydrate group.

Contributor(s)

Gjevestad GO, Holven KB, Rundblad A, Flatberg A, Myhrstad M, Karlsen K, Mutt SJ, Herzig K, Ottestad I, Ulven SM

Citation(s)

Submission date

Dec 09, 2019

Last update date

Mar 16, 2020

Contact name

Stine Marie Ulven

E-mail(s)

smulven@medisin.uio.no

Organization name

University of oslo

Department

Department of Nutrition

Street address

Sognsvannsveien 9

City

Oslo

ZIP/Postal code

0317

Country

Norway

Platforms (1)

Illumina HumanHT-12 V4.0 expression beadchip

Samples (62)

More...

GSM4210878	2_A3_visit 0 (baseline)
GSM4210879	2_A4_visit 1 (end of intervention)
GSM4210880	2_E5_visit 0 (baseline)

GSM4210881	2_E6_visit 1 (end of intervention)
GSM4210882	2_F3_visit 0 (baseline)
GSM4210883	2_F4_visit 1 (end of intervention)
GSM4210884	2_D3_visit 0 (baseline)
GSM4210885	2_D4_visit 1 (end of intervention)
GSM4210886	2_C3_visit 0 (baseline)
GSM4210887	2_C4_visit 1 (end of intervention)
GSM4210888	2_E3_visit 0 (baseline)
GSM4210889	2_E4_visit 1 (end of intervention)
GSM4210890	2_A5_visit 0 (baseline)
GSM4210891	2_A6_visit 1 (end of intervention)
GSM4210892	2_D7_visit 0 (baseline)
GSM4210893	2_D8_visit 1 (end of intervention)
GSM4210894	2_C5_visit 0 (baseline)
GSM4210895	2_C6_visit 1 (end of intervention)
GSM4210896	2_B9_visit 0 (baseline)
GSM4210897	2_B10_visit 1 (end of intervention)
GSM4210898	2_D5_visit 0 (baseline)
GSM4210899	2_D6_visit 1 (end of intervention)
GSM4210900	2_A7_ visit 0 (baseline)
GSM4210901	2_A8_visit 1 (end of intervention)
GSM4210902	2_A1_visit 0 (baseline)
GSM4210903	2_A2_visit 1 (end of intervention)
GSM4210904	2_B7_visit 0 (baseline)
GSM4210905	2_B8_visit 1 (end of intervention)
GSM4210906	2_E7_visit 0 (baseline)
GSM4210907	2_E8_ visit 1 (end of intervention)
GSM4210908	2_C7_visit 0 (baseline)
GSM4210909	2_C8_visit 1 (end of intervention)
GSM4210910	2_F7_visit 0 (baseline)
GSM4210911	2_F8_visit 1 (end of intervention)
GSM4210912	2_C9_visit 0 (baseline)
GSM4210913	2_C10_visit 1 (end of intervention)
GSM4210914	2_D9_visit 0 (baseline)
GSM4210915	2_D10_visit 1 (end of intervention)
GSM4210916	2_F9_visit 0 (baseline)
GSM4210917	2_F10_visit 1 (end of intervention)
GSM4210918	2_A11_visit 0 (baseline)
GSM4210919	2_A12_visit 1 (end of intervention)
GSM4210920	2_E9_visit 0 (baseline)
GSM4210921	2_E10_visit 1 (end of intervention)
GSM4210922	2_D11_visit 0 (baseline)
GSM4210923	2_D12_visit 1 (end of intervention)
GSM4210924	2_E11_visit 0 (baseline)
GSM4210925	2_E12_visit 1 (end of intervention)
GSM4210926	2_C11_visit 0 (baseline)
GSM4210927	2_C12_visit 1 (end of intervention)
GSM4210928	2_B11_visit 0 (baseline)
GSM4210929	2_B12_visit 1 (end of intervention)
GSM4210930	2_B1_visit 0 (baseline)
GSM4210931	2_B2_visit 1 (end of intervention)
GSM4210932	2_C1_visit 0 (baseline)
GSM4210933	2_C2_visit 1 (end of intervention)
GSM4210934	2_E1_visit 0 (baseline)
GSM4210935	2_E2_visit 1 (end of intervention)
GSM4210936	2_D1_visit 0 (baseline)
GSM4210937	2_D2_visit 1 (end of intervention)
GSM4210938	2_B3_visit 0 (baseline)
GSM4210939	2_B4_visit 1 (end of intervention)

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE141674_RAW.tar	26.2 Mb	(http)(custom)	TAR
GSE141674_non-normalized.txt.gz	17.3 Mb	(ftp)(http)	TXT
Processed data included within Sample table

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