|
| Status |
Public on Jan 28, 2020 |
| Title |
Expression data from p53 knocked-down human neuroepithelial stem (NES) cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study, we take advantage of human induced pluripotent stem (iPS) cell-derived neural stem cells to study the role of p53 during human brain development. We knocked down (KD) p53 in human neuroepithelial stem (NES) cells derived from iPS cells. Upon p53KD, NES cells rapidly show centrosome amplification and genomic instability. Gene expression analysis show downregulation of genes involved in oxidative phosphorylation (OXPHOS) upon loss of p53. In addition, p53KD neural stem cells upregulate genes involved in neuronal differentiation and display an increased pace of differentiating into neurons and exhibit a phenotype corresponding to more mature neurons compared to control neurons. Taken together, this demonstrates an important role for p53 in controlling genomic stability of neural stem cells and regulation of neuronal differentiation.
We used microarrays to identify gene expression changes in human NES cells upon p53 knockdown
|
| |
|
| Overall design |
NES cells were either transduced with lentivirus containing either Control or p53 shRNA construct. Transduced cells were selected by puromycin treatment to obtain NES cells with stable knock down of p53. Gene expression analysis was performed on RNA isolated from Control and p53 shRNA NES cells generated from three seperate lentiviral transductions.
|
| Web link |
https://doi.org/10.1038/s41419-019-2208-7
|
| |
|
| Contributor(s) |
Wilhelm M |
| Citation(s) |
31974372 |
| |
| Submission date |
Dec 13, 2019 |
| Last update date |
Jan 30, 2020 |
| Contact name |
Margareta T Wilhelm |
| E-mail(s) |
margareta.wilhelm@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
MTC
|
| Lab |
Biomedicum B7
|
| Street address |
Solnavägen 9
|
| City |
Stockholm |
| ZIP/Postal code |
17165 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL17692 |
[HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version] |
|
| Samples (6)
|
| GSM4217424 |
NES cells transduced with Control shRNA, biological replicate 1 |
| GSM4217425 |
NES cells transduced with Control shRNA, biological replicate 2 |
| GSM4217426 |
NES cells transduced with Control shRNA, biological replicate 3 |
| GSM4217427 |
NES cells transduced with p53 shRNA, biological replicate 1 |
| GSM4217428 |
NES cells transduced with p53 shRNA, biological replicate 2 |
| GSM4217429 |
NES cells transduced with p53 shRNA, biological replicate 3 |
|
| Relations |
| BioProject |
PRJNA595508 |
Less...
More...