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| Status |
Public on Aug 19, 2020 |
| Title |
Defining effects of in utero arsenic exposure on immune cells in lungs of adult mice exposed to Influenza A with single cell RNA sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our recent studies have shown that adult mice exposed to inorganic arsenic exclusively in utero (100 ppb via maternal drinking water) exhibit greater lung damage and inflammation following infection with IAV (Influenza A virus, H1N1) than control mice not exposed in utero. We employed an unbiased single cell RNA sequencing approach to conduct a high resolution molecular analysis of recruited immune cells and their transcriptional activities in the lungs of arsenic-exposed and control mice at day 3 after IAV infection. Transcriptional profiling of single immune cells from 6 mice (3 As, 3 Con, all exposed to IAV) revealed evidence of an enhanced inflammatory response mediated by dysregulation of innate immune functions of monocyte derived macrophages, neutrophils and alveolar macrophages.
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| Overall design |
Dams were exposed to 100ppb sodium arsenite or control drinking water from cervical plug formation until pups were born. Arsenic was removed from drinking water and F1 offspring were allowed to mature to adulthood (7-8 weeks). Three F1 adult female mice from each treatment group were randomly selected and infected with 0.5 LD50 of influenza A/PR8/34. Whole lung homogenate was collected at day 3 of infection, and CD45+ immune cells isolated for single cell RNA sequencing (10X Genomics).
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| Contributor(s) |
Hsu KS, Goodale BC, Ely KH, Hampton TH, Stanton BA, Enelow RI |
| Citation(s) |
32514536 |
| |
| Submission date |
Dec 14, 2019 |
| Last update date |
Aug 19, 2020 |
| Contact name |
Britton C Goodale |
| E-mail(s) |
Britton.C.Goodale@Dartmouth.edu
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| Organization name |
Geisel School of Medicine at Dartmouth
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| Department |
Dartmouth Cancer Center
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| Lab |
Leach
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| Street address |
1 Medical Center Drive
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| City |
Lebanon |
| State/province |
NH |
| ZIP/Postal code |
03756 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA595698 |
| SRA |
SRP237635 |
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