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| Status |
Public on Aug 12, 2020 |
| Title |
Effect of RAGE on macrophage transcriptome in regression of diabetic atherosclerosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ldlr null (Ager+/+ Ldlr-/-) diabetic mice were raised on a Western diet.
Their aortas were transplanted to diabetic mice rasied on standard chow,
which were either WT (Ager+/+ Ldlr+/+) or Ager null (Ager-/- Ldlr+/+). The
gene expression profile of either the donor (CD45.2) or recipient (CD45.1)
sorted macrophages from both genotypes was studied.
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| Overall design |
Ldlr-/- Donor: Macrophages from the aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and not transplanted (n=4)
WT Recipient: WT recipient macrophages, from the transplant: Aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and transplanted into standard chow-fed WT CD45.1 diabetic mice (n=4).
Ldlr-/- Donor → WT: Ldlr null donor macrophages, from the transplant: Aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and transplanted into standard chow-fed WT CD45.1 diabetic mice (n=4).
Ager-/- Recipient: Ager null recipient macrophages, from the transplant: Aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and transplanted into standart chow-fed Ager null CD45.1 diabetic mice (n=6).
Ldlr-/- Donor → Ager-/-: Ldlr null donor macrophages, from the transplant: Aortas of Ldlr null CD45.2 diabetic mice fed a Western Diet, and transplanted into standard chow-fed Ager null CD45.1 diabetic mice (n=4).
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| Contributor(s) |
Senatus L, López-Díez R, Egaña-Gorroño L, Liu J, Li H, Daffu G, Li Q, Rahman K, Vengrenyuk Y, Barrett T, Dewan Z, Friedman RA, Fisher EA, Ramasamy R, Schmidt AM |
| Citation(s) |
32641587 |
| Submission date |
Dec 17, 2019 |
| Last update date |
Aug 12, 2020 |
| Contact name |
Richard A Friedman |
| Organization name |
Columbia University Irving Medical Center
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| Department |
Medicine
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| Lab |
Cancer Center
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| Street address |
1130 St. Nicholas Av
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA596218 |
| SRA |
SRP237938 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142204_rage_regression_processed_data.txt.gz |
887.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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