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| Status |
Public on May 01, 2020 |
| Title |
Cardiac mesenchymal stromal cells from right and left ventricles display different biological properties. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Aims. We sought to analyze the physiological differences of human C-MSC between the left ventricle (LV) versus the right ventricle (RV) .
Methods and results. Autoptic cardiac specimens from LV and RV were obtained from healthy donors. Part was used for tissue sections; part was processed to obtain C-MSC. Isogenic LV and RV C-MSC were compared as for surface markers expression, cell proliferation/death ratio, differentiation capabilities and transcriptomics. Our results indicated a greater presence of C-MSC in RV vs. LV total tissue. Moreover, more cells can be obtained from RV than from LV after isolation procedures. LV and RV C-MSC are characterized by the expression of the same surface markers. Functional studies revealed RV-LV C-MSC comparable groth curves but higher apoptosis rate within the LV cells. Moreover, higher propensity of the RV to to collagen deposition was obseved. Transcriptome analysis confirmed differences in the expression of genes related to apoptosis regulation and extracellular matrix organization. Alongside, we unveiled further pathways specifically associated to LV or RV C-MSC.
Conclusions. These results contribute to understand the role of RV and LV C-MSC in physiological condition. Moreover, the obtained information can unravel their differential contribution to RV or LV- specific pathologic scenarios. RNA-Seq allowed identifying novel differentially expressed genes in RV vs. LV cells, which might uncover unappreciated mechanisms underlying ventricle-specific C-MSC regulation.
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| Overall design |
The poly(A)+ RNA profiles of 6 paired C-MSC samples obtained from the RV and 6 from the LV of the same human tissue were generated through RNA-Seq using the Ion GeneStudio S5 Prime System .
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| Contributor(s) |
Stadiotti I, Piacentini L, Vavassori C, Chiesa M, Micheli B, Guarino A, Polvani G, Colombo GI, Pompilio G, Sommariva E |
| Citation(s) |
32670081 |
| |
| Submission date |
Dec 17, 2019 |
| Last update date |
Jul 31, 2020 |
| Contact name |
Gualtiero Ivanoe Colombo |
| E-mail(s) |
gualtiero.colombo@cardiologicomonzino.it
|
| Phone |
+39 0258002464
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| Organization name |
Centro Cardiologico Monzino IRCCS
|
| Lab |
Immunology and Functional Genomics
|
| Street address |
Via Carlo Parea, 4
|
| City |
Milano |
| ZIP/Postal code |
20138 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA596219 |
| SRA |
SRP237939 |
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