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| Status |
Public on Sep 30, 2022 |
| Title |
RNA-seq analysis of DLD-1 cell lines expressing cDNAs of subunits on human mei-cohesin complexes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The study involves trabscriptome analysis using RNA-seq of stable transgenic DLD-1 cancer cell line, which express Tet-inducible combinations of mei-cohesin subunits.
The following combinations of mei-cohesin genes were expressed in DLD-1 : STAG3+SMC1beta, STAG3+SMC1beta+REC8, and STAG3+SMC1beta+RAD21L
The goal of the study was to investigate the RNA transcriptional response to the induction of mei-cohesin subunits in somatic cells using Tet-on inducible transgenes activated by an rtTA transgene and doxyciline.
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| Overall design |
The DLD-1 cell line, which is derived from adult male colorectal adenocarcinoma (ATCC® CCL-221™), were first stably infected with rtTA, Tet-On-STAG3 and Tet-On-SMC1beta lentifiruses This cell line was used as an additional control along with the parent cell line DLD-1. The SMC3+SMC1b cells were also separately infected with Tet-On-REC8 and Tet-On-RAD21L lentiviruses, to generate two triple transgenic cell lines modeling the somatic expression of two major meiotic cohesin complexes: REC8 and RAD21L-based. Both the drug-selected populations of transgenic cell lines and single clones were used as biological replicates.
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| Contributor(s) |
Boukaba A, Liu J, Wu Q, Liang J, Strunnikov A |
| Citation(s) |
36179046 |
| Submission date |
Dec 18, 2019 |
| Last update date |
Oct 03, 2022 |
| Contact name |
Alexander V. Strunnikov |
| E-mail(s) |
alexstrunnikov@gmail.com
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| Organization name |
GIBH
|
| Lab |
Molecular Epigenetics
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| Street address |
190 Kai Yuan Avenue
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platforms (1) |
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| Samples (12)
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| GSM4223679 |
STAG3_SMC1B_p1: 2x transgene population 1 |
| GSM4223680 |
STAG3_SMC1B_p2: 2x transgene population 2 |
| GSM4223681 |
STAG3_SMC1B_p3: 2x transgene population 3 |
| GSM4223682 |
STAG3_SMC1B_REC8_p1: 3x REC8 transgene population 1 |
| GSM4223683 |
STAG3_SMC1B_REC8_c2: 3x REC8 transgene clone 2 |
| GSM4223684 |
STAG3_SMC1B_REC8_c3: 3x REC8 transgene clone 3 |
| GSM4223685 |
STAG3_SMC1B_RAD21L_p1: 3x RAD21L transgene population 1 |
| GSM4223686 |
STAG3_SMC1B_RAD21L_c1: 3x RAD21L transgene clone 1 |
| GSM4223687 |
STAG3_SMC1B_RAD21L_c2: 3x RAD21L transgene clone 2 |
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| Relations |
| BioProject |
PRJNA596384 |
| SRA |
SRP238067 |
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