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Status |
Public on Dec 21, 2019 |
Title |
Single cell RNA sequencing analysis of the mouse colonic mesenchyme (Drop-seq) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Single cell RNA sequencing of the mouse colonic mesenchyme. Mesenchymal/lamina propria cells were isolated from the middle and distal colon of wild type mice in two biological replicates. For each biological replicate the colons of n = 2 mice were pooled. The vast majority of intestinal epithelial cells were depleted by Ethylenediaminetetraacetic acid (EDTA) treatment of the tissue samples and mesenchymal/lamina propria cells were isolated after enzymatic treatment with collagenase XI and dispase. Single cell RNA sequencing was performed with the Drop-seq protocol. N =5 Drop-seq collections (samples) were processed in total, two from the first biological replicate and three from the second.
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Overall design |
Two biological replicates (C1 and C2): one with two Drop-seq collections (samples C1A, C1B) and one with three Drop-seq collections (samples C2A, C2B, C2C)
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Contributor(s) |
Flavell RA, Roulis M |
Citation(s) |
32322056 |
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Submission date |
Dec 20, 2019 |
Last update date |
Apr 29, 2020 |
Contact name |
Richard Flavell |
E-mail(s) |
richard.flavell@yale.edu
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Organization name |
Yale University
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Department |
Department of Immunobiology
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Street address |
300 Cedar Street, TAC S-569
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City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06511 |
Country |
USA |
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Platforms (1) |
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Samples (5)
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This SubSeries is part of SuperSeries: |
GSE142431 |
Paracrine orchestration of intestinal tumorigenesis by a confined mesenchymal niche |
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Relations |
BioProject |
PRJNA597002 |
SRA |
SRP238352 |