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| Status |
Public on Aug 31, 2021 |
| Title |
The mRNA decay analysis of Plasmodium falciparum parasites with disrupted PfNot1.1 (PF3D7_1103800) at 24 hour post-invasion. |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by array
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| Summary |
The study aims to investigate functions of PfNOT1.1 in regulating mRNA decay in the malaria parasite, P. falciparum.
To achieve this goal, transcription was blocked by actinomycin D (actD) and the transcriptional profilling of parasites was analyzed by microarray technology.
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| Overall design |
The endogenous PfNot1.1 was disrupted. The transgenic line and the wildtype line were treated with actD to block transcription or DMSO as a control for four hours. Transcriptional profiling of initial parasites at 24 hpi, parasites treated with actD and parasites treated with DMSO was assessed by microarray.
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| Contributor(s) |
Liu Y, Bozdech Z, Featherstone M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 23, 2019 |
| Last update date |
Sep 02, 2021 |
| Contact name |
Ying Liu |
| E-mail(s) |
yliu037@e.ntu.edu.sg
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| Phone |
84030462
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| Organization name |
Nanyang Technological University
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| Department |
School of Biological Sciences
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| Lab |
Prof. Zbynek Bozdech's lab
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| Street address |
60 Nanyang Drive
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| City |
Singapore |
| ZIP/Postal code |
637551 |
| Country |
Singapore |
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| Platforms (1) |
| GPL11248 |
ZB/SBS-NTU Plasmodium falciparum 3D7 11.4K v1.1 |
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| Samples (12)
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| GSM4231334 |
PfNot1.1 knockout, initial, replicate 2 |
| GSM4231335 |
PfNot1.1 knockout, DMSO, replicate 2 |
| GSM4231336 |
PfNot1.1 knockout, actD, replicate 2 |
| GSM4231337 |
wildtype, initial, replicate 1 |
| GSM4231338 |
wildtype, DMSO, replicate 1 |
| GSM4231339 |
wildtype, actD, replicate 1 |
| GSM4231340 |
wildtype, initial, replicate 2 |
| GSM4231341 |
wildtype, DMSO, replicate 2 |
| GSM4231342 |
wildtype, actD, replicate 2 |
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| Relations |
| BioProject |
PRJNA597324 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142527_RAW.tar |
14.8 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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