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Status |
Public on Aug 31, 2021 |
Title |
The mRNA decay analysis of Plasmodium falciparum parasites with disrupted PfNot1.1 (PF3D7_1103800) at 24 hour post-invasion. |
Organism |
Plasmodium falciparum |
Experiment type |
Expression profiling by array
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Summary |
The study aims to investigate functions of PfNOT1.1 in regulating mRNA decay in the malaria parasite, P. falciparum. To achieve this goal, transcription was blocked by actinomycin D (actD) and the transcriptional profilling of parasites was analyzed by microarray technology.
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Overall design |
The endogenous PfNot1.1 was disrupted. The transgenic line and the wildtype line were treated with actD to block transcription or DMSO as a control for four hours. Transcriptional profiling of initial parasites at 24 hpi, parasites treated with actD and parasites treated with DMSO was assessed by microarray.
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Contributor(s) |
Liu Y, Bozdech Z, Featherstone M |
Citation missing |
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Submission date |
Dec 23, 2019 |
Last update date |
Sep 02, 2021 |
Contact name |
Ying Liu |
E-mail(s) |
yliu037@e.ntu.edu.sg
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Phone |
84030462
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Organization name |
Nanyang Technological University
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Department |
School of Biological Sciences
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Lab |
Prof. Zbynek Bozdech's lab
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Street address |
60 Nanyang Drive
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City |
Singapore |
ZIP/Postal code |
637551 |
Country |
Singapore |
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Platforms (1) |
GPL11248 |
ZB/SBS-NTU Plasmodium falciparum 3D7 11.4K v1.1 |
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Samples (12)
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GSM4231334 |
PfNot1.1 knockout, initial, replicate 2 |
GSM4231335 |
PfNot1.1 knockout, DMSO, replicate 2 |
GSM4231336 |
PfNot1.1 knockout, actD, replicate 2 |
GSM4231337 |
wildtype, initial, replicate 1 |
GSM4231338 |
wildtype, DMSO, replicate 1 |
GSM4231339 |
wildtype, actD, replicate 1 |
GSM4231340 |
wildtype, initial, replicate 2 |
GSM4231341 |
wildtype, DMSO, replicate 2 |
GSM4231342 |
wildtype, actD, replicate 2 |
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Relations |
BioProject |
PRJNA597324 |
Supplementary file |
Size |
Download |
File type/resource |
GSE142527_RAW.tar |
14.8 Mb |
(http)(custom) |
TAR (of GPR) |
Processed data included within Sample table |
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