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| Status |
Public on Dec 01, 2021 |
| Title |
small RNA sequencing of goat mamamary gland cells samples from cirRNA modification groups |
| Organism |
Capra hircus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The goat of this project is to explore cirRNA28250 efffect on small RNA to regulation goat mammary gland lipid metabolism. We tried to search the mechanism of cirRNA28250 regulation lipid metabolism through miRNA. small RNA seqencing of goat mamamary gland cells samples from different groups: 5NC, cirRNA28250 overexpression, 3NC,cirRNA28250 knockdown. The goat mammary gland cells were cultured in 3D condition. The cell were transfected with virus with cirRNA28250 gene (overexpression), or inhibition of cirRNA28250A expression (cirRNA28250 gene knockdown).
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| Overall design |
small RNA sequencing of goat mamamary gland cells samples from different groups: 5NC, cirRNA28250 overexpression, 3NC, cirRNA28250 knockdown. The goat mammary gland cells were cultured in 3D condition. The cell were transfected with virus with cirRNA28250 gene (overexpression), or inhibition of cirRNA28250 expression (cirRNA28250 gene knockdown).
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| Contributor(s) |
Zhao Y, Gao Y, Min L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 24, 2019 |
| Last update date |
Dec 01, 2021 |
| Contact name |
Yong Zhao |
| E-mail(s) |
yzhao818@hotmail.com
|
| Phone |
86-18661613617
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| Organization name |
Qingdao Agricultural University
|
| Department |
College of Life Sciences
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| Lab |
B604
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| Street address |
700 Changcheng Road
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| City |
Qingdao |
| State/province |
Shandong |
| ZIP/Postal code |
266109 |
| Country |
China |
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| Platforms (1) |
| GPL15473 |
Illumina HiSeq 2000 (Capra hircus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA597486 |
| SRA |
SRP238703 |
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