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Series GSE142654 Query DataSets for GSE142654
Status Public on Aug 30, 2021
Title Gene expression profiles of embryonic motor neurons in the SOD1G93A mouse model of amyotrophic lateral sclerosis: insights into earliest pathogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Although ALS typically presents in mid to late-life, there is increasing evidence for a protracted preclinical period of motor neuron dysfunction. The goal of this study is to identify the earliest gene expression patterns in lower motor neurons that drive selective neuronal vulnerability in a mouse model of ALS. We have implemented transgenic SOD1G93A with a lower motor neuron fluorescent reporter (HB9-GFP) mice to unambiguously isolate spinal motor neurons using FACS for gene expression profiling using RNA sequencing at embryonic day (E)12.5. Data from this transcriptomic study allowed the identification of disturbance in MN homeostasis which occur at the day of birth of spinal motor neurons in SOD1G93A mice. Pathway analysis uncovered several remarkably dysregulated pathways including RNA metabolism disturbance and excitotoxicity. Our findings have the potential to reveal insights into the incipient molecular mechanisms that confer motor neuron vulnerability and therefore may highlight relevant gene targets and pathways for effective intervention in ALS.
 
Overall design Gene expression profile of mouse spinal motor neurons. Wild-type control with 4 biological replicates compared with mouse SOD1G93A motor neurons 4 biological replicates at embryonic day 12.5.
 
Contributor(s) Zanganeh F
Citation(s) 35445192
Submission date Dec 27, 2019
Last update date Apr 27, 2022
Contact name fatemeh zanganeh
E-mail(s) fatemeh.zanganeh@florey.edu.au
Phone 0410275922
Organization name Florey
Lab MND lab
Street address 30 royal pde
City Parkville
State/province Victoria
ZIP/Postal code 3058
Country Australia
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM4237282 spinal motor neurons, WT-1
GSM4237283 spinal motor neurons, WT-2
GSM4237284 spinal motor neurons, WT-3
Relations
BioProject PRJNA597877
SRA SRP238900

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142654_E12_FeatureCount.txt.gz 2.2 Mb (ftp)(http) TXT
GSE142654_E12_Transcriptomics_DeSeq2.CSV.gz 47.5 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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