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| Status |
Public on May 04, 2009 |
| Title |
microRNA expression and opioid signaling |
| Platform organisms |
Caenorhabditis elegans; Drosophila melanogaster; Danio rerio; Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To study the relationship between microRNAs and μ-opioid receptor (MOR) signaling, we examined microRNA expression after chronic morphine or fentanyl treatment in rat primary hippocampal neurons and in mouse hippocampus. Mouse cerebellum region was also tested as a negative control to eliminate microRNA expression changes unrelated to MOR signaling, as the cerebellum is essentially devoid of MOR. We identified a number of microRNAs that altered their expression upon treatment with both morphine and fentanyl in the rat and mouse systems. There were, however, some microRNAs that changed in response to morphine, or fentanyl, but not both.
Keywords: Expression profiling
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| Overall design |
There are up to three biological replicates (indicated by 1, 2, and 3) of primary hippocampal neurons from new born rats and the cerebellum and hippocampus regions from adult mice treated for three days (control, morphine, and fentanyl). The biological replicates were from experiments performed on different dates. Each biological replicate contained cells or tissues collected from multiple animals so that enough RNA could be extracted for RNA analysis. RNA was labelled with a green dye, mixed with a reference DNA sample labelled with a red dye. The reference DNA contained a number of synthetic DNA oligos with mature microRNA sequences that served to verify microarray hybridization. RNA signals were in ch1, DNA signals ch2.
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| Contributor(s) |
Zeng Y, Zhang X, Zheng H, Law P |
| Citation(s) |
19854889 |
| Submission date |
Jan 02, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Xiaoxiao Zhang |
| E-mail(s) |
zhang688@umn.edu
|
| Organization name |
University of Minnesota
|
| Street address |
312 Church St. SE
|
| City |
Minneapolis |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (1) |
| GPL8021 |
Biomedical Genomics Center Microarray Facility Custom Multi-Species miRNA Microarray |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA111231 |
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