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| Status |
Public on Apr 13, 2020 |
| Title |
Histone demethylase SlJMJ6 promotes fruit ripening by removing H3K27 methylation of ripening-related genes in tomato [RNA-Seq] |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here, we found that the tomato jmjC domain-containing gene SlJMJ6 encodes a histone lysine demethylase that specifically demethylates H3K27 methylation. Overexpression of SlJMJ6 accelerated fruit ripening in tomato, which is associated with the up-regulated expression of a large number of ripening-related genes. Integrated analysis of RNA-seq and ChIP-seq data identified 55 genes that are targeted directly by SlJMJ6 and transcriptionally up-regulated with decreased H3K27m3 in SlJMJ6 overexpressing (SlJMJ6-OE) fruits. A large number of the SlJMJ6-regulated genes are involved in transcription regulation, ethylene biosynthesis, cell wall degradation, pigment biosynthesis, and hormone signaling. Fourteen ripening-related genes including RIN, ACS4, ACO1, PL, TBG4 were confirmed to be directly regulated by SlJMJ6 through removing H3K27me3. Taken together, these results indicated that SlJMJ6 is a ripening prompting H3K27me3 demethylase that activates the expression of the ripening-related genes by modulating H3K27me3, thereby facilitating fruit ripening in tomato. To our knowledge, this is the first report of the involvement of a histone lysine demethylase in the regulation of fruit ripening.
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| Overall design |
Tomato pericarp mRNA profiles of wild type (WT) and transgenic lines (JMJ6-OE) at 37 days after anthesis were generated by deep sequencing, in triplicate, using Illumina HiSeqTM 2000.
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| Contributor(s) |
Li Z, Jiang G, Liu X, Ding X, Zhang D, Wang X, Zhou Y, Yan H, Li T, Wu K, Jiang Y, Duan X |
| Citation(s) |
32255501 |
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| Submission date |
Dec 30, 2019 |
| Last update date |
Apr 13, 2020 |
| Contact name |
Li Zhiwei |
| E-mail(s) |
lizhiwei@scbg.ac.cn
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| Phone |
18825179606
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| Organization name |
South China Botanical Garden, Chinese Academy of Sciences
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| Lab |
Key Laboratory of Plant Resources Conservation and Sustainable Utilization
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| Street address |
No.723,Xingke Road
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| City |
Guangzhou |
| ZIP/Postal code |
518000 |
| Country |
China |
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| Platforms (1) |
| GPL25655 |
Illumina HiSeq 4000 (Solanum lycopersicum) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE142714 |
Histone demethylase SlJMJ6 promotes fruit ripening by removing H3K27 methylation of ripening-related genes in tomato |
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| Relations |
| BioProject |
PRJNA598113 |
| SRA |
SRP239046 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142713_WT-vs-J6.all.annot.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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