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| Status |
Public on Nov 01, 2021 |
| Title |
Single-cell RNA-seq analysis reveals reduction of B cells in the bone marrow promote fracture healing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Recent studies have identified B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation. However, it remains unknown how B cells participate in the healing of fractures. Here we find that B cells decrease significantly during fracture healing and returned to normal levels after that by single-cell profiling. Furthermore, we found that exosomes derived from B cells inhibited the differentiation of osteoblasts and osteoclasts in vitro. Additionally, injection of these exosomes in mice delayed fracture healing. Thus, maintaining low B cell levels in the bone marrow microenvironment promotes fracture healing.
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| Overall design |
scRNA profiles of fracture at different stage (control group, 30th day)
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| Contributor(s) |
Wang R, Tang H |
| Citation(s) |
34819916 |
| Submission date |
Dec 31, 2019 |
| Last update date |
Dec 07, 2021 |
| Contact name |
Renkai Wang |
| E-mail(s) |
jfsdfgjh@126.com
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| Organization name |
changhai hospital
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| Street address |
168 Changhai Road,
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| City |
shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA598334 |
| SRA |
SRP239180 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142786_AllSample.counts.tsv.gz |
15.3 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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