|
| Status |
Public on Sep 18, 2020 |
| Title |
RNA-seq of knocked-out oocytes and directly induced oocyte-like cells (DIOLs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
During female germline development oocytes become a highly specialized cell type, and form a maternal cytoplasmic store of crucial factors during oocyte growth. Oocyte growth is triggered at the primordial-primary follicle transition accompanied with dynamic changes in gene expression, yet the gene regulatory network underpinning oocyte growth remains elusive. Here we identified a set of transcription factors sufficient to trigger oocyte growth. By dissection of the change in gene expression and functional screening using an in vitro oocyte development system, we identified 8 transcription factors, each of which was essential for the primordial-primary follicle transition. Surprisingly, enforced expression of these transcription factors swiftly converted pluripotent stem cells to oocyte-like cells that were competent for fertilization and subsequent cleavage. These transcription factor-induced oocyte-like cells were formed without PGC specification, epigenetic reprogramming or meiosis, establishing that oocyte growth and lineage-specific de novo DNA methylation is separable from the preceding epigenetic reprogramming in PGCs. This study identifies a core set of transcription factors for orchestrating oocyte growth, and also provides an alternative source of ooplasm, which is a unique material for reproductive biology and medicine.
|
| |
|
| Overall design |
polyA+RNA was harvested from mouse oocytes and directly induced oocyte-like cells (DIOLs). Extracted polyA+RNA were used for the directional (strand-specific) RNA-seq library construction.
|
| |
|
| Contributor(s) |
Hayashi K, Hamazaki N |
| Citation(s) |
33328630 |
| |
| Submission date |
Jan 07, 2020 |
| Last update date |
Dec 21, 2020 |
| Contact name |
Nobuhiko Hamazaki |
| E-mail(s) |
hamazaki@hgs.med.kyushu-u.ac.jp
|
| Organization name |
Kyushu University
|
| Department |
Department of Developmental Stem Cell Biology
|
| Street address |
Maidashi 3-1-1, Higashi-ku,
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (71)
|
|
| This SubSeries is part of SuperSeries: |
| GSE143220 |
Directly induced oocyte-like Cells (DIOLs) |
|
| Relations |
| BioProject |
PRJNA599332 |
| SRA |
SRP239770 |
Less...
More...