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Series GSE143525 Query DataSets for GSE143525
Status Public on Jul 30, 2020
Title The Hepatocyte Clock and Feeding Interdependently Control Chrono-Homeostasis of Multiple Liver Cell Types (ATAC-seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Most cells in the body contain a cell autonomous molecular clock, but the requirement of peripheral clocks for circadian rhythmicity, and their effects on physiology, are not well understood. Here we show that deletion of core clock components REV-ERBa and b in adult mouse hepatocytes caused the loss of circadian rhythmicity of many liver genes, as expected, but also led to maintained and even gained rhythmicity of other genes without altering feeding behavior. The loss of REV-ERBs from hepatocytes leads to an exaggerated circadian rhythm of de novo lipogenesis and serum triglyceride levels. It is increasingly recognized that liver function is also influenced by non-hepatocytic cells, and remarkably the loss of REV-ERBs in hepatocytes remodeled the circadian transcriptomes of multiple cell types within the liver without altering their core clocks, indicating that hepatocytes communicated time signals to the non-hepatocytic cells. Finally, alteration of food availability, which is the dominant zeitgeber in the liver, demonstrated strong interdependence of the cell-autonomous hepatocyte clock mechanism and non-cell-autonomous environmental change. Together these studies reveal the interdependence of endogenous hepatocyte clocks and feeding entrainment on the regulation of circadian rhythms of multiple cell types in the liver.
Overall design RNA sequencing in livers, isolated EC and KC of control and adult hepatocytes REV-ERB a/b double knockout cell, ATAC-seq in isolated EC and KC of control and adult hepatocytes REV-ERB a/b double knockout cell, single nuclei RNA-seq in control and adult hepatocytes REV-ERB a/b double knockout cell
Contributor(s) Guan D, Xiong Y, Trin TM, Xiao Y, Hu W, Jiang C, Dierickx P, Jang C, Rabinowit JD, Lazar MA
Citation(s) 32732282
Submission date Jan 13, 2020
Last update date Oct 29, 2020
Contact name Dongyin Guan
Organization name Baylor College of Medicine
Lab Dongyin Guan
Street address 1 Baylor Plaza, ABBR
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (32)
GSM4261392 ATAC_seq_EC_ZT10_WT_rep1
GSM4261393 ATAC_seq_EC_ZT10_WT_rep2
GSM4261394 ATAC_seq_EC_ZT10_WT_rep3
This SubSeries is part of SuperSeries:
GSE143528 The hepatocyte clock and feeding control chronophysiology of multiple liver cell types
BioProject PRJNA600891
SRA SRP241718

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Supplementary file Size Download File type/resource
GSE143525_EC_ATAC_nfr.peak_unique.bed.gz 706.2 Kb (ftp)(http) BED
GSE143525_KC_ATAC_nfr.peak_unique.bed.gz 1.0 Mb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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