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| Status |
Public on Oct 06, 2021 |
| Title |
miRNA expression profiling and identification of a miRNA regulator for macrophage polarization in the murine alveolar macrophage cell line MH-S. |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Using the highly sensitive miRNA array, we screened differentially regulated miRNAs in IL-4-induced M2 macrophages and explored the functions of miR-378a-3p by Gene Ontology and Kyoto Encyclopedia of Genes annotation. Furthermore, the quantitative real-time polymerase chain reaction, western blot and dual luciferase reporter assay results showed that Grb2 is a specific target of miR-378a-3p, which will give new insights in disclosing the mechanism of macrophage polarization.
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| Overall design |
In this study, IL-4 induced miRNAs expression in murine alveolar macrophage cell line was measured at 48 hours after exposure to 20 ng/mL IL-4. Three independent experiments were performed.
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| Contributor(s) |
Wang Q, Jiang S |
| Citation(s) |
34589519 |
| |
| Submission date |
Jan 15, 2020 |
| Last update date |
Oct 07, 2021 |
| Contact name |
Qiujie Wang |
| E-mail(s) |
wangqiuj@mail2.sysu.edu.cn
|
| Organization name |
Sun Yat-sen University Second University Hospital
|
| Department |
Department of Respiratory Medicine
|
| Street address |
No.107 Yan jiang West Road
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510120 |
| Country |
China |
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| Platforms (1) |
| GPL18058 |
Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18, condensed Probe_ID version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA601470 |
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