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| Status |
Public on May 19, 2020 |
| Title |
Next Generation Sequencing of first trimester human cytotrophoblasts expressing a constitutively active mutant (5SA) of the transcriptional co-activator YAP. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Aim of the study was to elucidate YAP-regulated trophoblast-specific genes by analyzing differentially-expressed gene signatures between first trimester human cytotrophoblasts (vCTBs) transfected with a YAP-5SA mutant or a control plasmid.
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| Overall design |
To examine gene expression patterns of human trophoblasts expressing constitutively active YAP 5SA Next Generation Sequencing was performed. vCTBs, isolated from pooled placentas from 6-8th weeks of gestation, were transfected with pCMV-flag YAP2 -5SA or as control with pcDNA 3.1(-)plasmid DNA using electroporation following 24h cultivation on fibronectin-coated cell culture dishes. The analyses comprise in total 6 samples, 3 replicates of 3 individual trophoblast cell preparations.
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| Contributor(s) |
Meinhardt G, Knöfler M, Hetey S, Szilagyi A |
| Citation(s) |
32482863 |
| Submission date |
Jan 17, 2020 |
| Last update date |
Jun 04, 2020 |
| Contact name |
Martin Knöfler |
| E-mail(s) |
martin.knoefler@meduniwien.ac.at
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| Organization name |
Medical University of Vienna
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| Department |
Department of Obstetrics and Gynaecology, Reproductive Biology Unit
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| Street address |
Währinger Gürtel 18-20, 5Q,
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA601914 |
| SRA |
SRP242559 |
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