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Series GSE143883 Query DataSets for GSE143883
Status Public on Dec 31, 2020
Title Transgenerational epigenetic and transcriptomic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure in rat [Bisulfite-Seq]
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary In rats, direct exposure to TCDD causes myriad toxicities. Exposed rats experience hepatotoxicity, wasting syndrome and immune suppression, amongst others. “Inherited exposure”, as occurs in the F3 generation of directly exposed F0 animals, has also been shown to cause toxicity: both male and female F3 rats demonstrate an increased incidence of adult onset disease while males show increased incidence of kidney disease and an altered sperm epigenome. Here, we employ bisulfite-sequencing to explore the methylation profile of male F3 Sprague-Dawley rats bred through the paternal germ line from F0 dams exposed to a single dose of TCDD (0 or 1000 ng/kg body weight) by oral gavage. We identified multiple significant differentially methylated regions (DMRs) affecting receptor genes, including multiple olfactory receptors, as well as Egfr and Mc5r, typically with increased methylation among the TCDD-exposed lineage relative to control lineage.
Overall design On gestational day 11, groups of pregnant female Sprague-Dawley rats (n=6-8) were treated with a single dose of TCDD (0 or 1000 ng/kg bodyweight) dissolved in corn oil by oral gavage. Adult male F1 progeny were mated with untreated female rats to achieve F2 generations. The above procedure was repeated to further achieve F3 generations. At the end of the examination period, rats were euthanized by carbon dioxide exposure and subjected to tissue sampling. Testicular tissue was shipped on dry ice to the analytical laboratory for processing.
Contributor(s) Prokopec SD, Viluksela M, Miettinen HM, Boutros PC, Pohjanvirta R
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Submission date Jan 17, 2020
Last update date Jan 02, 2021
Contact name Stephenie Prokopec
Organization name Ontario Institute for Cancer Research
Street address 661 University Avenue, Suite 510
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
Platforms (1)
GPL20084 Illumina NextSeq 500 (Rattus norvegicus)
Samples (8)
GSM4276190 Male CornOil rep1 [Bisulfite-Seq]
GSM4276191 Male CornOil rep2 [Bisulfite-Seq]
GSM4276192 Male CornOil rep3 [Bisulfite-Seq]
This SubSeries is part of SuperSeries:
GSE143884 Transgenerational epigenetic and transcriptomic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure in rat
BioProject PRJNA601972
SRA SRP242585

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143883_RAW.tar 31.5 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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