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| Status |
Public on Jan 21, 2021 |
| Title |
RNA-sequencing of CD4+ T cells from mice prior to and after infection with influenza virus, and that were exposed developmentally to vehicle or TCDD |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used next generation RNAsequencing to identify how developmental exposure to TCDD alters gene expression in CD4+ T cells. Specifically, mice were developmentally exposed to vehicle or TCDD. At adulthood, CD4+ T cells were purified from peripheral lymph nodes of naive animals or the mediastinal lymph nodes of influenza A virus (IAV) infected mice (9 days post infection). RNA was isolated from these cells, and RNA-seq was performed. Gene expression analysis revealed developmental activation of the aryl hydrocarbon receptor with TCDD changes expression of genes that are involved in important CD4+ T cell functions.
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| Overall design |
Comparing gene expression patters of CD4+ T cells from naïve or IAV infected mice that were developmentally exposed to vehicle or TCDD. 3 biological replicates per sample.
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| Contributor(s) |
Burke CG, Myers JR, Lawrence BP |
| Citation(s) |
33449811 |
| |
| Submission date |
Jan 17, 2020 |
| Last update date |
Jan 21, 2021 |
| Contact name |
B. Paige Lawrence |
| E-mail(s) |
Paige_Lawrence@URMC.Rochester.edu
|
| Organization name |
University of Rochester
|
| Street address |
601 Elmwood Ave.
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14624 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA601990 |
| SRA |
SRP242613 |
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