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| Status |
Public on Jun 01, 2010 |
| Title |
Mouse Soleus Muscle Chronic Electrical Stimulation Study |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Stimulation of the mouse hindlimb via the sciatic nerve was used to induce contractions for 4 hours to investigate acute muscle gene activation in a model of muscle phenotype conversion. Initial force production (1.6 + 0.1 g/g body weight) declined 45% within 10 min and was maintained for the remainder of the experiment. Force returned to initial levels upon completion of the study. An immediate-early growth response was present in the EDL (FOS, JUN, ATF3, MAFK) with a similar but attenuated pattern in the soleus. Transcript profiles showed decreased fast fiber specific mRNA (myosin heavy chains 2A, 2B; troponins T3, I; alpha-tropomyosin, m-creatine kinase) and increased slow transcripts (myosin heavy chain slow/1beta, troponin C, tropomyosin 3gamma) in the EDL. Histological analysis of the EDL revealed glycogen depletion without inflammatory cell infiltration or myofiber damage in stimulated vs. control muscles. Several fiber type specific transcription factors (EYA1, TEAD1, NFATc1 and c4, PPARG, PPARGC1alpha and beta, BHLHB2) increased in the EDL along with transcription factors characteristic of embryogenesis (KLF4, SOX17, TCF15, PKNOX1, ELAV). No established in vivo satellite cell markers or the genes activated during our parallel studies of satellite cell proliferation in vitro (CYCLINS A2, B2, C, E1, MyoD) increased in the stimulated muscles. These data indicated that onset of fast to slow phenotype conversion occurred in the EDL within 4 hours of stimulation without satellite cell recruitment or muscle injury but was driven by phenotype specific transcription factors from resident fiber myonuclei including activation of nascent developmental transcriptional programs.
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| Overall design |
Adult male Swiss Webster mice (30-35 g) were anesthetized, a bipolar electrode was implanted adjacent to the sciatic nerve and the hindlimb immobilized. The voltage-force relation was determined to establish supramaximal stimulation conditions and the length-tension relation was determined to set the resting length for maximum twitch tension. Contractions were induced by sciatic nerve stimulation (0.5 msec duration, 2-5 volts). The muscles were allowed to rest 15 minutes for full metabolic recovery at physiologic temperatures. Supramaximal stimulation was applied at a rate of 10 Hz for 4 hours. At the end of each experiment the soleus muscles were carefully dissected and flash frozen in liquid nitrogen for analysis of mRNA expression via microarray analysis. The contralateral, unstimulated Soleus provided a genetically matched, paired control for each specimen.
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| Contributor(s) |
LaFramboise WA, Wiseman RW |
| Citation(s) |
19625612 |
| Submission date |
Jan 12, 2009 |
| Last update date |
Sep 18, 2012 |
| Contact name |
John Michael Krill-Burger |
| E-mail(s) |
burgerm@upmc.edu
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| Phone |
412-656-6727
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| Organization name |
University of Pittsburgh Medical Center
|
| Street address |
Rm. WG21.3 Shadyside Hospital
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15232 |
| Country |
USA |
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| Platforms (1) |
| GPL8063 |
GE Healthcare/Amersham Biosciences CodeLink™ Mouse Whole Genome Bioarray |
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| Samples (10)
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| GSM359752 |
Mouse 191 Control Contralateral Unstimulated Soleus Muscle |
| GSM359756 |
Mouse 191 Nerve Stimulated Soleus Muscle |
| GSM359757 |
Mouse 183 Control Contralateral Unstimulated Soleus Muscle |
| GSM359761 |
Mouse 183 Nerve Stimulated Soleus Muscle |
| GSM359762 |
Mouse 443 Control Contralateral Unstimulated Soleus Muscle |
| GSM359763 |
Mouse 443 Nerve Stimulated Soleus Muscle |
| GSM359764 |
Mouse 440 Nerve Stimulated Soleus Muscle |
| GSM359765 |
Mouse 440 Control Contralateral Unstimulated Soleus Muscle |
| GSM359766 |
Mouse 438 Nerve Stimulated Soleus Muscle |
| GSM359767 |
Mouse 438 Control Contralateral Unstimulated Soleus Muscle |
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| This SubSeries is part of SuperSeries: |
| GSE14421 |
Acute Molecular Response of Mouse Hindlimb Muscle To Chronic Stimulation |
|
| Relations |
| BioProject |
PRJNA114467 |
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