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Status |
Public on Jan 23, 2020 |
Title |
Goat's milk feeding enhances innate and adaptive immunities and alleviates allergen-induced airway inflammation in offspring mice |
Platform organism |
metagenomes |
Sample organism |
Mus musculus |
Experiment type |
Genome variation profiling by array
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Summary |
Goat’s milk (GM), as compared to Cow’s milk (CM), is easier for human to digest and absorb. GM feeding can improve colic, minor digestive disorders, asthma or eczema in infants. It has been demonstrated that GM have antioxidant and anti-inflammatory effects. Whether GM consumed in pregnant mothers has any immune-modulating effects on their newborns is still unclear. In this study, we used pregnant mothers and their offspring mice to verify this hypothesis. we collected feces of offspring before allergen sensitization (day 0) and two days after i.t. allergen challenge (day 16). Detection of cDNA of feces using Applied Biosystems™ Axiom™ Microbiome Array found the phyla of Bacteroidia, Clostridia, Flavobacteriia, Deferribacteres, Verrucomicrobiae and Gammaproteobacteria as well as some unclassified viruses. Comparison in ratio of Firmicutes to Bacteroidetes (F/B ratio), water-fed (W0) group had higher F/B ratio (0.79) than GM-fed (G0) (0.50) and CM-fed (C0) group (0.54) at Day 0. After HDM allergen sensitization and challenge, there was a remarkable increase of F/B ratio in water-fed mice (0.63 in WN vs. 0.84 in WA), while there was no change of F/B ratio in GM-fed (GN vs. GA) and CM-fed mice (CN vs. CA)
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Overall design |
44 total sample were analyzed
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Contributor(s) |
Kao H, Wang Y, Tzseng H, Shih-Hsin Wu L, Tsai H, Hsieh M, Chen P, Kuo W, Liu L, Liu Z, Wang J |
Citation missing |
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Submission date |
Jan 22, 2020 |
Last update date |
Jan 26, 2020 |
Contact name |
Jiu Yao Wang |
E-mail(s) |
a122@mail.ncku.edu.tw
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Organization name |
Nation Cheng Kung University
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Street address |
No.138,Sheng Li Road,Tainan
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City |
Tainan |
ZIP/Postal code |
Taiwan 704, R.O.C. |
Country |
Taiwan |
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Platforms (1) |
GPL28028 |
[Axiom_MDA] Applied Biosystems (Affymetrix) Axiom Microbiome Array |
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Samples (44)
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GSM4279714 |
stool at D0, cow's milk, naïve,rep2 |
GSM4279715 |
stool at D0, cow's milk, asthma,rep3 |
GSM4279716 |
stool at D0, cow's milk, naïve,rep3 |
GSM4279717 |
stool at D0, asthma,rep1 |
GSM4279718 |
stool at D0, naive,rep1 |
GSM4279719 |
stool at D0, naive,rep2 |
GSM4279720 |
stool at D0, asthma,rep2 |
GSM4279721 |
stool at D0, naive,rep3 |
GSM4279722 |
stool at D0, asthma,rep3 |
GSM4279723 |
stool at D16, cow's milk, asthma,rep1 |
GSM4279724 |
stool at D16, cow's milk, asthma,rep2 |
GSM4279725 |
stool at D16, cow's milk, naïve,rep1 |
GSM4279726 |
stool at D16, cow's milk, naïve,rep2 |
GSM4279727 |
stool at D16, cow's milk, asthma,rep3 |
GSM4279728 |
stool at D16, asthma,rep1 |
GSM4279729 |
stool at D16, naïve,rep1 |
GSM4279730 |
stool at D16, naïve,rep2 |
GSM4279731 |
stool at D16, asthma,rep2 |
GSM4279732 |
stool at D16, naïve,rep3 |
GSM4279733 |
stool at D16, asthma,rep3 |
GSM4279734 |
stool at D16, goat's milk, asthma,rep1 |
GSM4279735 |
stool at D16, goat's milk, naïve,rep1 |
GSM4279736 |
stool at D16, goat's milk, naïve,rep2 |
GSM4279737 |
stool at D16, goat's milk, asthma,rep2 |
GSM4279738 |
stool at D16, goat's milk, asthma,rep3 |
GSM4279739 |
stool at D16, goat's milk, naïve,rep3 |
GSM4279740 |
stool at D16, cow's milk, naïve,rep3 |
GSM4279741 |
naïve (mom),rep2 |
GSM4279742 |
naïve (mom),rep1 |
GSM4279743 |
stool goat's milk (mom),rep2 |
GSM4279744 |
stool goat's milk (mom),rep1 |
GSM4279745 |
stool at D0, naïve (mom),rep1 |
GSM4279746 |
stool at D0, naïve (mom),rep2 |
GSM4279747 |
2 stool at D0, cow's milk, asthma,rep1 |
GSM4279748 |
2 stool at D0, cow's milk, naïve,rep1 |
GSM4279749 |
stool at D0, goat's milk, naïve,rep2 |
GSM4279750 |
stool at D0, goat's milk, asthma,rep2 |
GSM4279751 |
stool at D0, goat's milk, asthma,rep3 |
GSM4279752 |
stool at D0, goat's milk, naïve,rep3 |
GSM4279753 |
cow's milk (mom),rep2 |
GSM4279754 |
cow's milk (mom),rep1 |
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Relations |
BioProject |
PRJNA602719 |
Supplementary file |
Size |
Download |
File type/resource |
GSE144086_RAW.tar |
376.6 Mb |
(http)(custom) |
TAR (of CEL, TXT) |
Processed data provided as supplementary file |
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