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Series GSE144106 Query DataSets for GSE144106
Status Public on Jun 30, 2021
Title RNA-sequencing in MCL cell lines after silencing cyclin D1
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Mantle cell lymphoma (MCL) is an aggressive B-cell neoplasm characterized by the t(11;14)(q13;q32) translocation leading to cyclin D1 overexpression. Cyclin D1 is a major cell cycle regulator and also has a role in transcription, but the effect of the latter in tumorigenesis remains largely unknown. Here, we investigated the transcriptional role of cyclin D1 in MCL and its impact on the pathogenesis of this neoplasm. Integrating genome-wide expression analysis of cyclin D1-silenced and overexpressing cells with cyclin D1 chromatin binding profiles, we identified a cyclin D1-activated transcriptional program in MCL cells.
 
Overall design We generated cyclin D1 silenced MCL cell lines (in Granta-519 and JeKo-1) by shRNA. We then performed RNA-sequencing experiments in shCyclinD1 (shCycD1) and control (shCtrl) cells to analyze the cyclin D1-dependent genome-wide expression modulation. Three biological replicates were included in the experiment.
 
Contributor(s) Demajo S, Castellano G, Jares P
Citation(s)
  • Demajo S, Albero R, Clot G, Castellano G et al. A Cyclin D1-Dependent Transcriptional Program Predicts Clinical Outcome in Mantle Cell Lymphoma. Clin Cancer Res 2021 Jan 1;27(1):213-225. PMID: 33046520
Submission date Jan 23, 2020
Last update date Jun 30, 2021
Contact name Santiago Demajo
E-mail(s) demajo@clinic.cat
Organization name IDIBAPS - Hospital Clínic Barcelona
Lab Molecular Pathology of Lymphoid Neoplasms
Street address Rosselló 153
City Barcelona
State/province Barcelona
ZIP/Postal code 08036
Country Spain
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM4280365 Granta shCtrl rep1
GSM4280366 Granta shCtrl rep2
GSM4280367 Granta shCtrl rep3
Relations
BioProject PRJNA602808
SRA SRP244245

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE144106_Granta_Matrix_RPKM.txt.gz 771.6 Kb (ftp)(http) TXT
GSE144106_Jeko_Matrix_RPKM.txt.gz 702.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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