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| Status |
Public on Jan 11, 2023 |
| Title |
LncRNA-mRNA expression profile and functional network of vascular dysfunction in endotoxemic rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
In our study, we used a rat model of LPS intervention to extract blood vessels. Differentially expressed LncRNA and mRNA were screened in lps rat blood vessels and normal rat blood vessels by microarray analysis. And through CNC and ceRNA analysis, lncRNA, miRNA, and mRNA regulatory network diagrams were constructed using verified lncRNA. This has the potential to highlight specific molecular functions and mechanisms. Later, we performed functional enrichment analysis and annotation to further explore the importance of lncRNA in vascular cell injury in sepsis. This work helps to reveal the underlying mechanism of vascular cell damage caused by sepsis and provides a new therapeutic target for controlling vascular damage in sepsis.
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| Overall design |
We use high-throughput technologies to screen the lncRNAs and mRNAs in the blood vessel tissues from LPS rats (n=5) compared with Ctrl rats (n=5). Differential expression lncRNAs and mRNAs were confirmed by qRT-PCR. We constructed a CNC and ceRNA network to reveal the core genes in hyposalivation.
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| Contributor(s) |
Xie H, Shen Z, Han Y, Wang Y |
| Citation(s) |
36611198 |
| |
| Submission date |
Jan 29, 2020 |
| Last update date |
Jan 12, 2023 |
| Contact name |
Jinghua Yang |
| E-mail(s) |
yangjh@xy-data.net
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| Organization name |
Beijing Zhiyun data technology co. LTD
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| Street address |
32 North Third Ring Road West, Haidian District, Beijing
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| City |
Beijing |
| ZIP/Postal code |
100098 |
| Country |
China |
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| Platforms (1) |
| GPL26961 |
Agilent-085985 Arraystar Rat lncRNA V3 microarray |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA603666 |
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