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| Status |
Public on Aug 05, 2020 |
| Title |
Contribution of brain pericytes in blood-brain barrier formation and maintenance: A transcriptomic study of cocultured human endothelial cells derived from hematopoietic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The formation, maintenance, and repair of the blood-brain barrier (BBB) are critical for central nervous system homeostasis. The interaction of endothelial cells with brain pericytes is known to induce BBB characteristics in brain endothelial cells during embryogenesis and could be used to differentiate human endothelial cells from stem cell source in in vitro BBB models. However, the molecular events involved in BBB maturation are not fully understood. To this end, human endothelial cells derived from hematopoietic stem cells were cultivated with either primary bovine or cell line-derived human brain pericytes to induce BBB formation. Subsequently, the transcriptomic profiles of solocultured vs. cocultured endothelial cells were analyzed over time by the Massive Analysis of cDNA Ends (MACE) technology. This RNA sequencing method is a 3’-end targeted, tag-based, reduced representation transcriptome profiling technique that can reliably quantify all polyadenylated transcripts including those with low expression. By analyzing the generated transcriptomic profiles, we can explore the molecular processes responsible for the functional changes observed in endothelial cells in coculture with brain pericytes (e.g. barrier tightening, changes in the expression of transporters and receptors). Our results identified several up- and downregulated genes and signaling pathways that provide a valuable data source to further delineate complex molecular processes that are involved in BBB formation and BBB maintenance. In addition, this data provides a source to identify novel targets for central nervous system drug delivery strategies.
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| Overall design |
27 samples were analysed by genome-wide gene expression profiling of solo- and cocultured ECs at 0, 24, 48 and 96 hours after putting ECs in coculture (3 biological replicates each consisting of 3 technical replicates) using the MACE method to identify differentially expressed genes upon pericyte introduction.
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| Contributor(s) |
Heymans M, Figueiredo R, Dehouck L, Francisco D, Sano Y, Shimizu F, Kanda T, Bruggmann R, Winter P, Gosselet F, Culot M |
| Citation(s) |
32723387 |
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| Submission date |
Jan 29, 2020 |
| Last update date |
Aug 05, 2020 |
| Contact name |
Maxime Culot |
| Organization name |
Université d'Artois
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| Lab |
Laboratoire de la Barrière Hémato-Encéphalique (LBHE)
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| Street address |
Rue Jean Souvraz - SP 18
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| City |
Lens |
| ZIP/Postal code |
62307 |
| Country |
France |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (27)
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| GSM4288901 |
Solo-culture, Timepoint 48, Replicate 1 |
| GSM4288902 |
Solo-culture, Timepoint 48, Replicate 2 |
| GSM4288903 |
Solo-culture, Timepoint 48, Replicate 3 |
| GSM4288904 |
Solo-culture, Timepoint 96, Replicate 1 |
| GSM4288905 |
Solo-culture, Timepoint 96, Replicate 2 |
| GSM4288906 |
Solo-culture, Timepoint 96, Replicate 3 |
| GSM4288907 |
HumanPericytes coculture, Timepoint 24, Replicate 1 |
| GSM4288908 |
HumanPericytes coculture, Timepoint 24, Replicate 2 |
| GSM4288909 |
HumanPericytes coculture, Timepoint 24, Replicate 3 |
| GSM4288910 |
HumanPericytes coculture, Timepoint 48, Replicate 1 |
| GSM4288911 |
HumanPericytes coculture, Timepoint 48, Replicate 2 |
| GSM4288912 |
HumanPericytes coculture, Timepoint 48, Replicate 3 |
| GSM4288913 |
HumanPericytes coculture, Timepoint 96, Replicate 1 |
| GSM4288914 |
HumanPericytes coculture, Timepoint 96, Replicate 2 |
| GSM4288915 |
HumanPericytes coculture, Timepoint 96, Replicate 3 |
| GSM4288916 |
BovinePericytes coculture, Timepoint 24, Replicate 1 |
| GSM4288917 |
BovinePericytes coculture, Timepoint 24, Replicate 2 |
| GSM4288918 |
BovinePericytes coculture, Timepoint 24, Replicate 3 |
| GSM4288919 |
BovinePericytes coculture, Timepoint 48, Replicate 1 |
| GSM4288920 |
BovinePericytes coculture, Timepoint 48, Replicate 2 |
| GSM4288921 |
BovinePericytes coculture, Timepoint 48, Replicate 3 |
| GSM4288922 |
BovinePericytes coculture, Timepoint 96, Replicate 1 |
| GSM4288923 |
BovinePericytes coculture, Timepoint 96, Replicate 2 |
| GSM4288924 |
BovinePericytes coculture, Timepoint 96, Replicate 3 |
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| Relations |
| BioProject |
PRJNA603751 |
| SRA |
SRP245871 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE144474_all_rawcount_merged.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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