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| Status |
Public on Jun 01, 2020 |
| Title |
Metabolic flexibility of SUP05 under low DO growth conditions |
| Organism |
Candidatus Thioglobus autotrophicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Chemoautotrophic bacteria from the SUP05 clade often dominate anoxic waters in marine oxygen minimum zones (OMZs) where reduced sulfur can fuel carbon fixation and denitrification. Some members of the SUP05 clade are facultative aerobes that thrive at the boundaries of OMZs where they experience fluctuations in dissolved oxygen (DO).
The degree to which SUP05 contribute to nitrate reduction in these regions depends on their sensitivity to oxygen. We evaluated growth and quantified differences in gene expression in Ca. T. autotrophicus strain EF1 from the SUP05 clade under high DO (22 μM), anoxic, and low DO (3.8 μM) concentrations.
We show that strain EF1 cells respire oxygen and nitrate and that cells have higher growth rates, express more genes, and fix more carbon when oxygen becomes available for aerobic respiration.
Evidence that facultatively aerobic SUP05 are more active and respire nitrate when oxygen becomes available at low concentrations suggests that they are an important source of nitrite across marine OMZ boundary layers.
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| Overall design |
mRNA profiles of a pure culture of Ca. T. autotrophicus strain EF1 growing under three different dissolved oxygen concentrations, anoxic, low DO and high DO, were generated in duplicate by Illumina Miseq high-throughput sequencing
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| Web link |
https://ami-journals.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2F1462-2920.15226&file=emi15226-sup-0001-supinfo.docx
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| Contributor(s) |
Mattes TE, Ingalls AE, Burke S, Morris RM |
| Citation(s) |
32893469 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P42 ES013661 |
Airborne PCBs: Sources, Exposures, Toxicities, Remediation |
THE UNIVERSITY OF IOWA |
Keri C Hornbuckle |
|
| Submission date |
Jan 29, 2020 |
| Last update date |
Oct 27, 2023 |
| Contact name |
Timothy E Mattes |
| E-mail(s) |
tim-mattes@uiowa.edu
|
| Phone |
319-335-5065
|
| Organization name |
University of Iowa
|
| Street address |
4105 Seamans Center
|
| City |
Iowa City |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL28093 |
Illumina MiSeq (Candidatus Thioglobus autotrophicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA603769 |
| SRA |
SRP245881 |
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