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| Status |
Public on Jan 12, 2010 |
| Title |
Genome-wide direct targets of PIL5 (ChIP-chip analysis of PIL5) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
PIL5 is a key negative regulator of phytochrome mediated seed germination and PIL5 protein is degraded by red light irradiation through phytochrome.
The ChIP-chip analysis aimed to find various PIL5 direct targets in the Arabidopsis genome.
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| Overall design |
PIL5-myc over-expressed seeds were used for ChIP-chip analysis. Conventional ChIP DNA was amplified by using LM-PCR method. Input DNA (before immuno-precipitation) was used as control DNA.Total three biological replicates were used for the ChIP-chip analysis.
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| |
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| Contributor(s) |
Choi G |
| Citation(s) |
19244139 |
| |
| Submission date |
Jan 15, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Giltsu Choi |
| E-mail(s) |
gchoi@kaist.edu
|
| Phone |
82423502636
|
| Fax |
82423502610
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| Organization name |
KAIST
|
| Department |
Biological Sciences
|
| Lab |
Choi Laboratory
|
| Street address |
335 Gwahangno
|
| City |
Daejeon |
| ZIP/Postal code |
305-701 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL8080 |
Nimblegen Arabidopsis thaliana Whole Genome Tiling Array (385K) 3 of 3 |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA111783 |
