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| Status |
Public on Jan 20, 2023 |
| Title |
RNA-Seq analysis of gene expression changes by wildtype and Nlrx1 deficient BEAS-2B airway epithelial cells in response to A. fumigatus viable conidia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Aspergillus fumigatus is an opportunistic fungal pathogen of the respiratory system that may cause invasive infection or an allergic response. Bronchial airway epithelial cells compromise the most abundant cell type of the pulmonary-air interface. Our recent findings suggest loss of Nlrx1 by BEAS-2B airway epithelial cells result in a hyper inflammatory response as well as decreased conidial processing. We challenged wildtype Nlrx1 deficient BEAS-2B cells with viable A. fumigatus AF293 conidia to identify early response differentially expressed genes and pathways between the two cell populations.
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| Overall design |
Wildtype and Nlrx1 deficient BEAS-2B cells were challenged with viable Aspergillus fumigatus conidia for 0 and 3 hours. Total RNA was extracted prior to challenge (0 hrs) and 3 hours post challenge.
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| Contributor(s) |
Kale SD, Kastelberg B |
| Citation missing |
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| |
| Submission date |
Feb 03, 2020 |
| Last update date |
Jan 20, 2023 |
| Contact name |
Shiv D. Kale |
| E-mail(s) |
sdkale@vt.edu
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| Phone |
540-231-3784
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| Organization name |
Virginia Tech
|
| Department |
Biocomplexity Institute
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| Street address |
1015 Steger Hall, Life Science Circle
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| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA604500 |
| SRA |
SRP246800 |
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