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| Status |
Public on Mar 14, 2020 |
| Title |
Expression data from FACS-isolated lung endothelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the role of a TIE1-function blocking antibody on gene expression of lung endothelial cells.
Lung endothelial cells were FACS-isolated from primary tumor-bearing mice. Mice were treated either with IgG or TIE1-blocking antibody during primary tumor growth. Total RNA were used for microarray analysis using Clariom S mouse assays (Applied Biosystems).
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| Overall design |
LLC primary tumors were treated with IgG or TIE1-blocking antibody. Lung EC were FACS-isolated. Total RNA was isolated with Arcturus PicoPure RNA isolation kit. cDNA libraries were prepared and analyzed by the DKFZ Genomics core facility, according to the standard operating protocols.
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| Contributor(s) |
Mahak S, Hellmut A |
| Citation(s) |
32302470 |
| |
| Submission date |
Feb 06, 2020 |
| Last update date |
Jun 13, 2020 |
| Contact name |
Mahak Singhal |
| E-mail(s) |
m.singhal@dkfz.de
|
| Organization name |
German Cancer Research Center (DKFZ)
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (8)
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| GSM4299276 |
IgG-treated, biological replicate 4 |
| GSM4299277 |
anti-TIE1 treated, biological replicate 1 |
| GSM4299278 |
anti-TIE1 treated, biological replicate 2 |
| GSM4299279 |
anti-TIE1 treated, biological replicate 3 |
| GSM4299280 |
anti-TIE1 treated, biological replicate 4 |
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| Relations |
| BioProject |
PRJNA605154 |
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