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| Status |
Public on Sep 30, 2010 |
| Title |
microRNA and mRNA expression profiles of human pancreatic islet-like cell clusters |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Type 1 diabetes is an autoimmune destruction of pancreatic islet beta cell disease, and it is important to find new alternative source of the islet beta cells to replace the damaged cells. Human embryonic stem (hES) cells possess unlimited self-renewal and pluripotency and thus have the potential to provide an unlimited supply of different cell types for tissue replacement. The hES-T3 cells with normal female karyotype were first differentiated into embryoid bodies and then induced to generate the pancreatic islet-like cell clusters, which expressed pancreatic islet cell-specific markers of insulin, glucagon and somatostatin. The expression profiles of microRNAs and mRNAs from the pancreatic islet-like cell clusters were further analyzed and compared with those of undifferentiated hES-T3 cells and differentiated embryoid bodies. MicroRNAs negatively regulate the expression of protein-coding mRNAs. The pancreatic islet-like cell clusters were found to exhibit very high expression of microRNAs miR-186, miR-199a and miR-339, which down-regulated the expression of LIN28, PRDM1, CALB1, GCNT2, RBM47, PLEKHH1, RBPMS2 and PAK6. Therefore, these microRNAs are very likely to play important regulatory roles in the differentiation of pancreatic islet cells and early embryonic development.
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| Overall design |
In this investigation, both miRNA and mRNA expression profiles from the pancreatic islet-like cell clusters differentiated from hES-T3 cells (T3pi) were quantitatively determined and compared with those of undifferentiated hES-T3 cells grown on mouse embryonic fibroblast (MEF) feeder (T3ES) and embryoid bodies differentiated from hES-T3 cells (T3EB). Several target genes of pancreatic islet cell-specific miRNAs were identified.
***This submission represents the mRNA expression component of the study only***
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| Contributor(s) |
Chen B, Yu S, Singh S, Li SS |
| Citation(s) |
20735361 |
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| Submission date |
Jan 21, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Sung-Liang Yu |
| E-mail(s) |
slyu@ntu.edu.tw
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| Phone |
886-2-23958341
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| Organization name |
National Taiwan University
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| Department |
Clinical Laboratory Sciences and Medical Biotechnology
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| Lab |
Microarray Core Facility
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| Street address |
Jen Ai Road Section1
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| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (7)
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| GSM239824 |
Human embryonic stem cell line T3ES, biological rep1 |
| GSM239825 |
Human embryonic stem cell line T3ES, biological rep2 |
| GSM239826 |
Human embryonic stem cell line T3ES, biological rep3 |
| GSM239827 |
hES-T3 derived embryoid bodies, biological rep1 |
| GSM239828 |
hES-T3 derived embryoid bodies, biological rep2 |
| GSM362248 |
Human pancreatic islet-like cell clusters, biological rep1 |
| GSM362249 |
Human pancreatic islet-like cell clusters, biological rep2 |
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| Relations |
| BioProject |
PRJNA111645 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14503_RAW.tar |
34.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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