|
| Status |
Public on Dec 31, 2023 |
| Title |
Proteomics of centromere repeat in distinct chromatin states reveals pathways to heterochromatin nucleation and transmission |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Heritable heterochromatin domain formation is initiated by dynamic changes in the proteome on specialized loci, leading to chromatin modifier recruitment, heterochromatin assembly and propagation. Here we characterize the proteomes associated with a defined heterochromatin nucleation region in three distinct chromatin states: before de novo H3K9 methylation nucleation, following heterochromatin assembly and upon heterochromatin disassembly. Our analyses separate sequence-dependent from sequence-independent processes, providing a systematic examination of the template-driven nucleation and epigenetic wiring underlying heterochromatin transmission. We identify functional interactions that reveal an integral role for the conserved nucleolytic Grc3 complex, and its associated network, as pioneer factors in heterochromatin assembly. More broadly, we demonstrate the power of quantitative proteomics-based strategies for the unbiased scrutiny of the single regions assembled in distinct chromatin states.
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| |
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| Overall design |
wt-phet & wt_pcontrol H3K9me2 ChIP-seq
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| |
|
| Contributor(s) |
Allshire R, Auchynnikava T, White SA, Torres-Garcia S, Tong P |
| Citation missing |
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| |
| Submission date |
Feb 11, 2020 |
| Last update date |
Dec 31, 2023 |
| Contact name |
Robin Allshire |
| E-mail(s) |
Robin.Allshire@ed.ac.uk
|
| Organization name |
The University of Edinburgh
|
| Lab |
Allshire Lab
|
| Street address |
Max Born Cresent
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH9 3BF |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL25575 |
Illumina MiniSeq (Schizosaccharomyces pombe) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA605953 |
| SRA |
SRP248155 |
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