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| Status |
Public on Mar 10, 2021 |
| Title |
Transcriptional analysis of naïve and central memory CD4 and CD8 T cells from healthy subjects treated with IL-27 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To examine the broad impact of IL-27 on human T lymphocytes, we performed a microarray analysis assessing >20,000 well annotated genes on purified naïve (CD45RA+CD45RO-CCR7+) and central memory (CD45RA-CD45RO+CCR7+) CD4+ and CD8+ T cells from three healthy donors that were activated in vitro (plate bound anti-CD3 and soluble anti-CD28) in the presence or absence of human recombinant IL-27 (100 ng/mL).
Our goal was to investigate the impact of interleukin-27 on the gene expression profil of human CD4 and CD8 T lymphocytes.
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| Overall design |
Naive and central memory CD4 and CD8 T cells from 3 healthy donors were sorted and then stimulated for 3 days with αCD3-αCD28 in the presence or not of IL-27 (100 ng/mL). RNA was purified and transcriptome-wide analysis of gene expression was performed by Genome Québec using the Clariom S HT array.
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| Contributor(s) |
Clénet ML, Arbour N |
| Citation(s) |
33728050 |
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https://doi.org/10.1002/cti2.1262
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| Submission date |
Feb 14, 2020 |
| Last update date |
Mar 23, 2021 |
| Contact name |
Nathalie Arbour |
| E-mail(s) |
nathalie.arbour@umontreal.ca
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| Organization name |
CHUM-University of Montreal
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| Department |
Neuroscience
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| Street address |
900 Saint Denis Street
|
| City |
Montréal |
| State/province |
Quebec |
| ZIP/Postal code |
H2X0A9 |
| Country |
Canada |
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| Platforms (1) |
| GPL24324 |
[Clariom_S_Human_HT] Affymetrix Clariom S Assay HT, Human (Includes Pico Assay) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA606778 |
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