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| Status |
Public on Feb 18, 2020 |
| Title |
An exon DNA element modulates heterochromatin spreading in the master regulator for sexual commitment in malaria parasites |
| Organism |
Plasmodium falciparum |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Heterochromatin is essential in all eukaryotic systems to maintain genome integrity, long-term gene repression and to help chromosome segregation during mitosis. However, heterochromatin regions must be restricted by boundary elements to avoid its spreading over actively transcribed loci. In Plasmodium falciparum, facultative heterochromatin is important to regulate parasite virulence, antigenic variation and transmission, but the underlying molecular mechanisms that regulate repressive regions remain unknown. To investigate this topic, we chose the ap2-g gene, that forms a strictly delimited heterochromatin island of 10 kb with no adjacent repressive chromatin. Using electrophoretic motility shift assay (EMSA) we identified an ap2-g exon element at the 3? end that shifts in the presence of nuclear extracts. Upon replacement of this region by a gfp gene, we observed a shift in the heterochromatin boundary, resulting in HP1 to spread over ~2 additional kb downstream. We used this DNA element to affinity purify candidate proteins followed by liquid-chromatography tandem mass-spectrometry (LC-MS/MS). The identified complexes are enriched in RNA-binding proteins, pointing to a potential role of RNA in the regulation of the ap2-g heterochromatin 3? boundary. Our results provide insight into the unexplored topic of heterochromatin boundary biology in P. falciparum and identifies a DNA element in the master regulator of sexual commitment that modulates heterochromatin spreading.
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| |
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| Overall design |
Examination of HP1 distribution in wild type and 2 clones from a mutant line.
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| Contributor(s) |
Cordon-Obras C, Barcons-Simon A, Scheidig-Benatar C, Claës A, Sabatet V, Loew D, Scherf A |
| Citation missing |
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| |
| Submission date |
Feb 17, 2020 |
| Last update date |
Mar 10, 2020 |
| Contact name |
Carlos Cordon-Obras |
| E-mail(s) |
obsidian1983@gmail.com
|
| Organization name |
Instituto Parasitologia Lopez Neyra
|
| Street address |
Av del Conocimiento
|
| City |
Granada |
| ZIP/Postal code |
18016 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL21298 |
Illumina NextSeq 500 (Plasmodium falciparum) |
|
| Samples (20)
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|
| Relations |
| BioProject |
PRJNA607036 |
| SRA |
SRP249694 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145378_3prime_RI_B3_HP1_1.FE.bedgraph.gz |
144.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145378_3prime_RI_B3_HP1_2.FE.bedgraph.gz |
123.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145378_3prime_RI_E1_HP1_1.FE.bedgraph.gz |
136.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145378_3prime_RI_E1_HP1_2.FE.bedgraph.gz |
129.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145378_A11_HP1_1_FE.bigWig |
140.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE145378_A11_HP1_2_FE.bigWig |
135.4 Mb |
(ftp)(http) |
BIGWIG |
| GSE145378_E1_HP1_1_FE.bigWig |
140.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE145378_E1_HP1_2_FE.bigWig |
124.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE145378_H8_HP1_1_FE.bigWig |
120.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE145378_H8_HP1_2_FE.bigWig |
77.1 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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