|
| Status |
Public on May 01, 2020 |
| Title |
The NtrYX two-component system regulates the bacterial cell envelope |
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To investigate the R. sphaeroides NtrYX two component system on RNA transcript levels, we used global RNA sequencing (RNA-seq) to compare transcript abundance in parent and DntrYX strains grown under aerobic conditions. Our previous studies were performed in strains deficient at synthesizing the polyhydroxybutyrate, so we used strains D0382 and DNtrYXD0382, herein referred to as parent and DntrYX, respectively. We also used ChIP-seq to identify NtrX binding sites throughout the genome.
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| |
|
| Overall design |
Comparing ∆ntrYX to parent strain for RNA-seq and using ChIP-seq to map NtrX binding sites
|
| |
|
| Contributor(s) |
Lemmer KC, Alberge F, Dohnalkova AC, Myers KS, Schaub RE, Lenz JD, Imam S, Dillard JP, Noguera DR, Donohue TJ |
| Citation(s) |
32430476 |
| |
| Submission date |
Feb 18, 2020 |
| Last update date |
May 21, 2020 |
| Contact name |
Kevin S Myers |
| E-mail(s) |
kmyers2@wisc.edu
|
| Organization name |
University of Wisconsin - Madison
|
| Department |
Great Lakes Bioenergy Research Center
|
| Street address |
5127 WEI, 1552 University Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53726 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL18842 |
Illumina HiSeq 2500 (Rhodobacter sphaeroides 2.4.1) |
| GPL28141 |
Illumina NextSeq 500 (Rhodobacter sphaeroides 2.4.1) |
|
| Samples (11)
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|
| Relations |
| BioProject |
PRJNA607228 |
| SRA |
SRP249799 |
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