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| Status |
Public on May 25, 2010 |
| Title |
Gene expression profiling of tumor stroma of MCF7 breast cancer xenografts overexpressing podoplanin (mouse array) |
| Platform organism |
Mus musculus |
| Sample organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Expression of the membrane glycoprotein podoplanin is upregulated in several human cancers and might be associated with their malignant progression. The exact biological function and molecular targets of cancer cell-expressed podoplanin have remained unclear, however. Here, we ectopically overexpressed podoplanin in a human breast carcinoma xenograft model to study its role in cancer progression. To identify potential molecular mediators of podoplanin-induced effects in the murine tumor stroma we compared transcriptional profiles of podoplanin-overexpressing and control tumors using mouse microarrays.
Keywords: comparative transcriptional profiling
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| Overall design |
MCF7 human breast carcinoma cells were stably transfected with the pTet-Off-regulator plasmid and the pTRE2hyg response plasmid, comprising or not full-length cDNA of human podoplanin (transcript variant 1, GenBank Accession number NM_006474). Three podoplanin-overexpressing and three control clones were isolated and each of them was injected bilaterally into the mammary fat pads of five female nude mice. Tumors were harvested after 24 days of growth and RNA was extracted from each of them. RNA of the 10 tumors derived from the same cell clone was pooled to give a total of six samples for microarray analysis (3x podoplanin-overexpressing, 3x control).
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| Contributor(s) |
Cueni LN, Detmar M |
| Citation(s) |
20616339 |
| |
| Submission date |
Jan 23, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Leah N Cueni |
| E-mail(s) |
leah.cueni@bluewin.ch
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| Organization name |
ETH Zurich
|
| Department |
Institute of Pharmaceutical Sciences
|
| Street address |
Wolfgang Pauli-Str. 10
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| City |
Zurich |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL2995 |
ABI Mouse Genome Survey Microarray |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE14552 |
Gene expression profiling of MCF7 breast cancer xenografts overexpressing podoplanin |
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| Relations |
| BioProject |
PRJNA114391 |
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