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| Status |
Public on Oct 12, 2023 |
| Title |
small RNAs sequencing in sweet sorghum under cadmium stress |
| Organism |
Sorghum bicolor |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Four small RNA libraries from two contrasting sweet sorghum genotypes were sequenced. In this study, One hundred and ninety-five conserved miRNAs belonging to 56 families and 25 putative novel miRNAs from 28 precursors were identified, among which 38 conserved and 24 novel miRNAs were differentially expressed under Cd stress and/or between H18 and L69. Two groups of them: miR169p/q-nov_23 and miR408 were further focused through the coexpression analysis and might be involved in Cd transport, cytoskeleton activity and cell wall construction by regulating their targets. This study presents new insights into the regulatory roles of miRNAs in Cd accumulation and tolerance in sweet sorghum and will help to develop high-Cd accumulation or high Cd-resistant germplasm of sweet sorghum through molecular breeding and/or genetic engineering approaches.
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| Overall design |
Four sRNA libraries were constructed from the roots of H18 and L69 (high-Cd accumulation one H18, and low-Cd accumulation one L69) treated with 10 µM CdCl2 for 24 hours, using the plants without CdCl2 treatment as control (named H18-CK, H18-Cd, L69-CK and L69-Cd, respectively). High throughput sequencing of miRNAs was then performed.
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| Contributor(s) |
Li Y, Jia W, Feng J, Lin K, Lv S, Jiang P, Zhang X, Yi Z, Wang D, Guo Z |
| Citation(s) |
34185181 |
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| Submission date |
Feb 20, 2020 |
| Last update date |
Jan 11, 2024 |
| Contact name |
YinXin Li |
| E-mail(s) |
yxli@ibcas.ac.cn
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| Organization name |
Institute of Botany, Chinese Academy of Sciences
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| Street address |
Nanxin Cun 20th, Xiangshan, Haidian district
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| City |
Beijing |
| ZIP/Postal code |
100093 |
| Country |
China |
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| Platforms (1) |
| GPL21615 |
Illumina HiSeq 2500 (Sorghum bicolor) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA607748 |
| SRA |
SRP250137 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145617_RAW.tar |
123.0 Mb |
(http)(custom) |
TAR (of FA) |
| GSE145617_total_TPM.xls.gz |
2.1 Kb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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