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| Status |
Public on Aug 31, 2020 |
| Title |
Chronic Inflammation Prediction for Inhaled Particles, the Impact of Material Cycling and Quarantining in the Lung Epithelium |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We are daily exposed to a multitude of health hazardous airborne particulate matter with notable deposition in the fragile alveolar region of our lungs. Hence, there is a great need for identification and prediction of material-associated diseases, currently hindered due to the lack of in-depth understanding of causal relationships, in particular between acute exposures and chronic symptoms. By applying advanced microscopies and omics to in vitro and in vivo systems, together with in silico molecular modelling, we have here determined that the long-lasting response to a single exposure can originate from the interplay between the newly discovered nanomaterial quarantining and nanomaterial cycling between different lung cell types. This new insight finally allows us to predict the spectrum of lung inflammation associated with materials of interest using only in vitro measurements and in silico modelling potentially relating outcomes to material properties for large number of materials thus boosting safe-by-design-based material development. Because of its profound implications for animal-free predictive toxicology, our work paves the way to a more efficient and hazard-free introduction of numerous new advanced materials into our lives.
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| Overall design |
We performed gene expression microarray analysis of LA-4 cells, MH-S cells or cocultures treated with TiO2 nanotubes (for 4h or 48h) or controls (for 0h or 48h)
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| Contributor(s) |
Majaron H, Kokot B, Sebastijanović A, Voss C, Podlipec R, Zawilska P, Berthing T, Ballester C, Danielsen PH, Contini C, Ivanov M, Krišelj A, Čotar P, Zhou Q, Ponti J, Zhernovkov V, Schneemilch M, Pušnik M, Umek P, Pajk S, Joubert O, Schmid O, Urbančič I, Irmler M, Beckers J, Lobaskin V, Halappanavar S, Quirke N, Lyubartsev A, Vogel U, Koklič T, Stöger T, Štrancar J |
| Citation(s) |
33073368 |
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| Submission date |
Feb 27, 2020 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
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| Organization name |
Helmholtz Zentrum Muenchen
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| Department |
Institute of Experimental Genetics
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| Street address |
Ingolstaedter Landstr. 1
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| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA609104 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE146036_RAW.tar |
51.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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