|
| Status |
Public on Jun 01, 2020 |
| Title |
A negative reciprocal regulatory axis between cyclin D1 and HNF4α modulates cell cycle progression and metabolism in the liver |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
This study examines the regulation of HNF4a by cyclin D1 in hepatic cell lines and mouse liver.
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| Overall design |
Three different cell lines were treated with control of cyclin D1 siRNA, followed by RNA-seq. Mice had transduction of cyclin D1 (or a truncation mutant called cyclin D1-RD) into the liver using recombinant adenovirus, followed by HNF4a ChIP-seq.
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| Contributor(s) |
Wu H, Reizel Y, Wang YJ, Lapiro JL, Kren BT, Schug J, Rao S, Morgan A, Herman A, Shekels LL, Rassette M, Lane AN, Cassel T, Fan TM, Manivel JC, Gunewardena SS, Apte U, Sicinski P, Kaestner K, Albrecht JH |
| Citation(s) |
32631996 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DK054921 |
Cyclin D1/CDK4 Complex in Hepatocyte Proliferation |
UNIVERSITY OF MINNESOTA |
JEFFREY H ALBRECHT |
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| |
| Submission date |
Feb 27, 2020 |
| Last update date |
Aug 31, 2020 |
| Contact name |
Jeffrey H Albrecht |
| E-mail(s) |
albre010@umn.edu
|
| Organization name |
University of Minnesota
|
| Department |
Medicine - Gastroenterology
|
| Street address |
One Veterans Drive
|
| City |
Miinneapolis |
| State/province |
MN |
| ZIP/Postal code |
55417 |
| Country |
USA |
| |
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| Platforms (3) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (198)
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| Relations |
| BioProject |
PRJNA609166 |
| SRA |
SRP251094 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE146053_ADV-CyclinD1-FLAG-HA_HNF4a_ChIP_Rep1.peaks.txt.gz |
838.5 Kb |
(ftp)(http) |
TXT |
| GSE146053_ADV-CyclinD1-FLAG-HA_HNF4a_ChIP_Rep2.peaks.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE146053_ADV-CyclinD1-FLAG-HA_HNF4a_ChIP_Rep3.peaks.txt.gz |
753.8 Kb |
(ftp)(http) |
TXT |
| GSE146053_ADV-D1-RD-FLAG_HNF4a_ChIP_Rep1.peaks.txt.gz |
955.3 Kb |
(ftp)(http) |
TXT |
| GSE146053_ADV-D1-RD-FLAG_HNF4a_ChIP_Rep2.peaks.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE146053_ADV-D1-RD-FLAG_HNF4a_ChIP_Rep3.peaks.txt.gz |
1016.2 Kb |
(ftp)(http) |
TXT |
| GSE146053_Control-ADV_HNF4a_ChIP_Rep1.peaks.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE146053_Control-ADV_HNF4a_ChIP_Rep2.peaks.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE146053_Control-ADV_HNF4a_ChIP_Rep3.peaks.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE146053_RawTranscriptCounts_AML12.xls.gz |
2.5 Mb |
(ftp)(http) |
XLS |
| GSE146053_RawTranscriptCounts_HEPG2.xls.gz |
1.8 Mb |
(ftp)(http) |
XLS |
| GSE146053_RawTranscriptCounts_SkHep1.xls.gz |
2.8 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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